药学与临床研究
藥學與臨床研究
약학여림상연구
PHARMACEUTICAL AND CLINICAL RESEARCH
2013年
1期
21-25
,共5页
林勤剑%江伟健%韩丽萍%石磊%赵树进
林勤劍%江偉健%韓麗萍%石磊%趙樹進
림근검%강위건%한려평%석뢰%조수진
眼镜蛇毒%神经病理性疼痛%谷氨酸-天冬氨酸转运体%脊神经结扎
眼鏡蛇毒%神經病理性疼痛%穀氨痠-天鼕氨痠轉運體%脊神經結扎
안경사독%신경병이성동통%곡안산-천동안산전운체%척신경결찰
目的:观察椎管内注射眼镜蛇毒najanalgesin对大鼠L5脊神经结扎并剪断(spinal nerve ligation and transection,SNL)术后脊髓谷氨酸-天冬氨酸转运体GLAST (glutamate-asparate transporter,GLAST) mRNA表达的影响,探讨najanalgesin脊髓镇痛机制.方法:采用L5SNL模拟神经病理性疼痛,von Frey hair测定大鼠术侧后足50％机械刺激撤足反射阈值(50％paw withdrawal threshold,PWT)判断模型成功,real-time PCR检测SNL 7 d后脊髓L4-L6GLAST mRNA表达.结果:SNL诱导大鼠产生了机械痛敏,表现为50％机械刺激PWT降低,模型复制成功.SNL 7d后脊髓GLAST mRNA表达降低,椎管内给予najanalgesin剂量相关上调SNL大鼠脊髓GLAST mRNA的表达.结论:Naj analgesin可以增加脊髓GLAST mRNA的表达,可能是其脊髓镇痛机制之一.
目的:觀察椎管內註射眼鏡蛇毒najanalgesin對大鼠L5脊神經結扎併剪斷(spinal nerve ligation and transection,SNL)術後脊髓穀氨痠-天鼕氨痠轉運體GLAST (glutamate-asparate transporter,GLAST) mRNA錶達的影響,探討najanalgesin脊髓鎮痛機製.方法:採用L5SNL模擬神經病理性疼痛,von Frey hair測定大鼠術側後足50％機械刺激撤足反射閾值(50％paw withdrawal threshold,PWT)判斷模型成功,real-time PCR檢測SNL 7 d後脊髓L4-L6GLAST mRNA錶達.結果:SNL誘導大鼠產生瞭機械痛敏,錶現為50％機械刺激PWT降低,模型複製成功.SNL 7d後脊髓GLAST mRNA錶達降低,椎管內給予najanalgesin劑量相關上調SNL大鼠脊髓GLAST mRNA的錶達.結論:Naj analgesin可以增加脊髓GLAST mRNA的錶達,可能是其脊髓鎮痛機製之一.
목적:관찰추관내주사안경사독najanalgesin대대서L5척신경결찰병전단(spinal nerve ligation and transection,SNL)술후척수곡안산-천동안산전운체GLAST (glutamate-asparate transporter,GLAST) mRNA표체적영향,탐토najanalgesin척수진통궤제.방법:채용L5SNL모의신경병이성동통,von Frey hair측정대서술측후족50％궤계자격철족반사역치(50％paw withdrawal threshold,PWT)판단모형성공,real-time PCR검측SNL 7 d후척수L4-L6GLAST mRNA표체.결과:SNL유도대서산생료궤계통민,표현위50％궤계자격PWT강저,모형복제성공.SNL 7d후척수GLAST mRNA표체강저,추관내급여najanalgesin제량상관상조SNL대서척수GLAST mRNA적표체.결론:Naj analgesin가이증가척수GLAST mRNA적표체,가능시기척수진통궤제지일.
