中医临床研究
中醫臨床研究
중의림상연구
CLINICAL JOURNAL OF CHINESE MEDICINE
2013年
10期
11-11,14
,共2页
罗秋燕%王苏%李卫林%申素芳
囉鞦燕%王囌%李衛林%申素芳
라추연%왕소%리위림%신소방
人胎羊膜%红花注射液%切创愈合%转化生长因子-β
人胎羊膜%紅花註射液%切創愈閤%轉化生長因子-β
인태양막%홍화주사액%절창유합%전화생장인자-β
Human fetal amniotic membrane%Safflower injection%Wound healing%TGF-β
目的:研究红花注射液保存人胎羊膜对大鼠皮肤切创愈合中TGF-β表达的影响。方法:建立大鼠皮肤切创模型,应用免疫组织化学技术,通过计算机图像分析系统检测红花注射液保存人胎羊膜对大鼠皮肤组织中TGF-β表达的影响。结果:切创后2d、3d,红花+羊膜组的TGF-β积分光密度明显低于对照组、羊膜组、红花组(P<0.05);切创后5d、7d,四组的TGF-β表达无统计学差异(P>0.05)。结论:结果表明经红花注射液保存后的人胎羊膜在大鼠皮肤切创愈合的早期阶段(2d~3d),可减少创缘周围TGF-β的表达;促进创口的愈合、减少瘢痕形成。
目的:研究紅花註射液保存人胎羊膜對大鼠皮膚切創愈閤中TGF-β錶達的影響。方法:建立大鼠皮膚切創模型,應用免疫組織化學技術,通過計算機圖像分析繫統檢測紅花註射液保存人胎羊膜對大鼠皮膚組織中TGF-β錶達的影響。結果:切創後2d、3d,紅花+羊膜組的TGF-β積分光密度明顯低于對照組、羊膜組、紅花組(P<0.05);切創後5d、7d,四組的TGF-β錶達無統計學差異(P>0.05)。結論:結果錶明經紅花註射液保存後的人胎羊膜在大鼠皮膚切創愈閤的早期階段(2d~3d),可減少創緣週圍TGF-β的錶達;促進創口的愈閤、減少瘢痕形成。
목적:연구홍화주사액보존인태양막대대서피부절창유합중TGF-β표체적영향。방법:건립대서피부절창모형,응용면역조직화학기술,통과계산궤도상분석계통검측홍화주사액보존인태양막대대서피부조직중TGF-β표체적영향。결과:절창후2d、3d,홍화+양막조적TGF-β적분광밀도명현저우대조조、양막조、홍화조(P<0.05);절창후5d、7d,사조적TGF-β표체무통계학차이(P>0.05)。결론:결과표명경홍화주사액보존후적인태양막재대서피부절창유합적조기계단(2d~3d),가감소창연주위TGF-β적표체;촉진창구적유합、감소반흔형성。
Objective: To investigate the effect of TGF-β expression on amniotic membrane saved by safflower during wound healing of rats. Methods: Establishing rats model of skin incised wound and using the immunohistochemical techniques, the effect of TGF-β expression on amniotic membrane saved by safflower during wound healing was detected by computer image analysis system. Results:After 2d and 3d, the expression of TGF-βintegral optical in amniotic membrane saved by Safflower was significantly lower than the control group, amniotic group and the safflower group (P<0.05);There was no significant difference among the four groups after 5d and 7d (P>0.05). Conclusion:The results show that human fetal amniotic saved by safflower injection can reduce the TGF-βexpression in the early stages of skin wound healing in rats (2d~3d), but also could promote wound healing and reduce scar formation.