CAJ | 학술논문

背景：最新系列研究发现低浓度尼古丁具有促进血管生成，加速皮肤伤口愈合的作用，但低浓度尼古丁能否在颌面部软组织创伤修复中尤其是口腔黏膜创伤修复中发挥作用目前还不清楚。 　　目的：观察低浓度尼古丁对大鼠硬腭黏膜缺损修复的影响。 　　方法：Wistar大鼠65只，于硬腭第一磨牙近中至第三磨牙远中的黏膜作一直径为3 mm的圆形切创缺损。造模后局部给药，大鼠随机分成低浓度尼古丁+凝胶组、凝胶组、空白对照组，每组动物分别在3，7，10，14 d处死，苏木精-伊红染色观察组织愈合过程，并通过大体标本结合图像测量分析比较各组间伤口愈合差异。 　　结果与结论：修复后3 d各组间愈合情况差异无显著性意义；修复后7，10 d组低浓度尼古丁+凝胶组愈合快于凝胶组和空白对照组(P <0.05)；修复后14 d各组创面已基本愈合，各组间差异无显著性意义。提示低浓度尼古丁可能具有促进大鼠硬腭膜缺损修复的作用。
배경：최신계렬연구발현저농도니고정구유촉진혈관생성，가속피부상구유합적작용，단저농도니고정능부재합면부연조직창상수복중우기시구강점막창상수복중발휘작용목전환불청초。 　　목적：관찰저농도니고정대대서경악점막결손수복적영향。 　　방법：Wistar대서65지，우경악제일마아근중지제삼마아원중적점막작일직경위3 mm적원형절창결손。조모후국부급약，대서수궤분성저농도니고정+응효조、응효조、공백대조조，매조동물분별재3，7，10，14 d처사，소목정-이홍염색관찰조직유합과정，병통과대체표본결합도상측량분석비교각조간상구유합차이。 　　결과여결론：수복후3 d각조간유합정황차이무현저성의의；수복후7，10 d조저농도니고정+응효조유합쾌우응효조화공백대조조(P <0.05)；수복후14 d각조창면이기본유합，각조간차이무현저성의의。제시저농도니고정가능구유촉진대서경악막결손수복적작용。
BACKGROUND:Low concentration of nicotine promotes the angiogenesis and facilitates the healing of skin wounds. However, the role of low concentration of nicotine on the repair of maxil ofacial soft tissue trauma especial y oral mucosa stil remains unclear OBJECTIVE:To evaluate the effect of low concentration of nicotine on mucosa defect repair of rat hard palate. METHODS:A circular soft tissue defect at 3 mm diameter was produced in the centre of hard palate of 65 Wistar rats. After the operation, animals were randomly divided into low concentration of nicotine with gel group, gel group and control group. Rats were sacrificed at 3, 7, 10 and 14 days post-surgery. The wound healing was detected with hematoxylin-eosin staining and the difference of wound healing in different groups was compared with gross observation and image measurement. RESULTS AND CONCLUSION:There was no significant difference in the wound healing in different groups on day 3 post-surgery. On days 7 and 10, the group of low concentrations of nicotine with gel was faster than gel group and control group (P<0.05);the wounds were completely healed on day 14, with no significant difference among the groups. Low concentrations of nicotine may promote the mucosa defects repair of rat hard palate.