CAJ | 학술논문

背景：长链非编码 RNA 调控一系列生理过程，被认为在发育、分化和代谢的基因调控中发挥重要的作用。MC3T3-E1、C2C12和 C3H10T1/2细胞可向骨细胞、肌细胞等多个方向分化，用于肌肉骨骼等运动系统相关疾病的研究。 　　目的：观察长链非编码RNA在骨形态发生蛋白2诱导成骨分化中的作用。 　　方法：对MC3T3-E1、C2C12和C3H10T1/2细胞在骨形态发生蛋白2诱导下，成骨分化过程中的长链非编码RNA表达变化进行芯片分析，找到在3株细胞中同时变化的长链非编码RNA，siRNA干扰方法观察长链非编码RNA对骨形态发生蛋白2诱导成骨分化的影响，采用Real-Time PCR与碱性磷酸酶染色检测成骨相关指标。 　　结果与结论：骨形态发生蛋白2诱导MC3T3-E1、C2C12和C3H10T1/2成骨分化过程中，相应成骨指标增高，成肌指标肌细胞生成素降低。筛选出骨形态发生蛋白2诱导成骨分化过程中出发挥作用的长链非编码RNA AK007000。AK007000被干扰后成骨分化指标碱性磷酸酶、骨钙素、RUNX2、SP7表达下降，肌细胞生成素表达上升。因此，AK007000可能具有促进成骨抑制成肌作用。
배경：장련비편마 RNA 조공일계렬생리과정，피인위재발육、분화화대사적기인조공중발휘중요적작용。MC3T3-E1、C2C12화 C3H10T1/2세포가향골세포、기세포등다개방향분화，용우기육골격등운동계통상관질병적연구。 　　목적：관찰장련비편마RNA재골형태발생단백2유도성골분화중적작용。 　　방법：대MC3T3-E1、C2C12화C3H10T1/2세포재골형태발생단백2유도하，성골분화과정중적장련비편마RNA표체변화진행심편분석，조도재3주세포중동시변화적장련비편마RNA，siRNA간우방법관찰장련비편마RNA대골형태발생단백2유도성골분화적영향，채용Real-Time PCR여감성린산매염색검측성골상관지표。 　　결과여결론：골형태발생단백2유도MC3T3-E1、C2C12화C3H10T1/2성골분화과정중，상응성골지표증고，성기지표기세포생성소강저。사선출골형태발생단백2유도성골분화과정중출발휘작용적장련비편마RNA AK007000。AK007000피간우후성골분화지표감성린산매、골개소、RUNX2、SP7표체하강，기세포생성소표체상승。인차，AK007000가능구유촉진성골억제성기작용。
BACKGROUND:Long non-coding RNA (lncRNA) regulates a series of physiological processes and it is considered to play important roles in the gene regulation of development, differentiation and metabolism. MC3T3-E1, C2C12 and C3H10T1/2 cells are able to differentiate into different celllineages, such as bone cells and muscle cells, and they can be used in the study of musculoskeletal diseases. OBJECTIVE:To study the role of lncRNA in osteogenic differentiation induced by bone morphogenetic protein 2. METHODS:Osteogenic differentiation of MC3T3-E1, C2C12 and C3H10T1/2 cells was induced by bone morphogenetic protein 2, and microarray expression profiling of lncRNA was undertaken in osteogenic differentiation. LncRNA simultaneous changes in three cells were found out. The siRNA interference of the lncRNA was used to study its effects on the osteogenic differentiation induced by bone morphogenetic protein 2. Real-time PCR and alkaline phosphatase staining were applied to detect osteogenesis related indicators. RESULTS AND CONCLUSION:In the process of osteogenic differentiation induced by bone morphogenetic protein 2, osteogenic differentiation indicators were increased, while myogenic differentiation indicator myogenin was reduced. LncRNA AK007000 was screened out to play a role in osteogenic differentiation induced by bone morphogenetic protein 2. Knockdown of lncRNA AK007000 decreased the expression of osteogenic differentiation indicators, while increased the expression of myogenin. Therefore, AK007000 may play a role in promoting osteogenic differentiation and inhibiting myogenic differentiation.