CAJ | 학술논문

背景：牙齿缺失导致牙槽嵴骨质的改建和持续吸收，严重影响种植体植入的条件和种植区软硬组织的美观。目的：评价纳米羟基磷灰石/聚己内酯电纺支架促进即刻种植体周围骨缺损的成骨效果。方法：制作犬骨髓间充质干细胞复合纳米羟基磷灰石/聚己内酯电纺支架的组织工程化骨。拔除6只实验犬双侧下颌第二前磨牙，在其近中根牙槽窝处制备种植床，即刻植入种植体，在钛钉颊侧制作三壁骨缺损，两侧骨缺损处分别植入组织工程化骨与Bio-Oss小牛无机骨粉，并在材料表面覆盖Bio-Gide胶原膜。术后即刻、4周、8周、12周X射线测量种植体周围骨灰度值；12周后完整取出下颌骨，甲苯胺蓝染色观察骨缺损区的微观结构，新生骨量、形态结构及种植体-骨结合情况。结果与结论：两组间术后各时间点骨密度变化无明显差异，表明两组材料在促进骨再生过程中的成骨效果基本一致。组织工程化骨组骨缺损区内形成致密板状骨，可见成熟骨细胞，哈弗氏管，新生骨-种植体结合较紧密；Bio-Oss小牛无机骨粉组板层骨致密，新骨中有少量Bio-Oss颗粒分布，成骨细胞较组织工程化骨组少，部分哈弗管结构内可见到毛细血管，新骨与植入材料之间形成桥形连接，与种植体结合紧密。表明纳米羟基磷灰石/聚己内酯支架复合骨髓间充质干细胞、Bio-Gide胶原膜可促进种植体周围牙槽骨再生。
배경：아치결실도치아조척골질적개건화지속흡수，엄중영향충식체식입적조건화충식구연경조직적미관。목적：평개납미간기린회석/취기내지전방지가촉진즉각충식체주위골결손적성골효과。방법：제작견골수간충질간세포복합납미간기린회석/취기내지전방지가적조직공정화골。발제6지실험견쌍측하합제이전마아，재기근중근아조와처제비충식상，즉각식입충식체，재태정협측제작삼벽골결손，량측골결손처분별식입조직공정화골여Bio-Oss소우무궤골분，병재재료표면복개Bio-Gide효원막。술후즉각、4주、8주、12주X사선측량충식체주위골회도치；12주후완정취출하합골，갑분알람염색관찰골결손구적미관결구，신생골량、형태결구급충식체-골결합정황。결과여결론：량조간술후각시간점골밀도변화무명현차이，표명량조재료재촉진골재생과정중적성골효과기본일치。조직공정화골조골결손구내형성치밀판상골，가견성숙골세포，합불씨관，신생골-충식체결합교긴밀；Bio-Oss소우무궤골분조판층골치밀，신골중유소량Bio-Oss과립분포，성골세포교조직공정화골조소，부분합불관결구내가견도모세혈관，신골여식입재료지간형성교형련접，여충식체결합긴밀。표명납미간기린회석/취기내지지가복합골수간충질간세포、Bio-Gide효원막가촉진충식체주위아조골재생。
BACKGROUND:Alveolar bone remodeling and sustained absorption due to tooth extraction seriously affect the implanting conditions and morphology of hard and soft tissue in implant zone. OBJECTIVE:To evaluate the effect of nano-hydroxyapatite/polycaprolactone electrospinning scaffolds to improve the osteogenic effect of bone defects around immediate implants. METHODS:Tissue-engineered bone was prepared by combining canine bone marrow mesenchymal stem cels with nano-hydroxyapatite/polycaprolactone electrospinning scaffold. Bilateral mandibular second premolars from six dogs were extracted mandibular second premolar, and an immediate implant was placed in the mesial fossa of the mandibular second premolar. Three-wal bone defects was made buccaly using titanium nails, then tissue-engineered bone and Bio-Oss bone powders were implanted bilateraly covered by colagen membranes (Bio-Gide). Imageology examination was performed to measure bone gray levels immediately, 4, 8, 12 weeks after surgery. After 12 weeks, the mandible was removed completely, toluidine blue staining was used for observation of microstructure, new bone formation, bone morphology and implant osseointegration. RESULTS AND CONCLUSION: Between the two groups, there was no difference in bone mineral density at each time point after surgery, indicating that the effects of the two materials to promote bone regeneration process are basicaly the same. After implantation, the dense lamelar bone formed in the bone defect region of tissue-engineered bone group, mature bone cels, Haversian canal, and implant osseointegration were visible. While, in the Bio-Oss group, the lamelar bone was dense, a smal amount of Bio-Oss particles distributed within new bone tissues, fewer bone cels were found, a part of Haversian canal was shown to have blood capilaries, and new bone was in close conjunction with the implant. These findings indicate that the nano-hydroxyapatite/polycaprolactone electrospinning scaffold combined with bone marrow mesenchymal stem cels and Bio-Gide colagen membrane can promote the regeneration of alveolar bone around the implant.