CAJ | 학술논문

背景：血管成形后再狭窄严重限制了经皮冠状动脉介入治疗的应用和远期疗效。平滑肌细胞的表型转变，增殖是血管成形后再狭窄的重要机制。 　　目的：探讨利用球囊在体转导骨桥蛋白短发夹状RNA，通过抑制实验性动脉粥样硬化模型兔损伤血管部位骨桥蛋白的表达，预防血管成形后再狭窄。 　　方法：构建动脉粥样硬化模型兔20只，随机等分成空质粒组和OPN-shRNA质粒组，分别利用球囊在腹主动脉导入OPN-shRNA质粒和空载体。 　　结果与结论：2组兔球囊扩张后血管平滑肌层出现特异性绿色荧光，且随转染后时间的延长荧光强度逐渐降低，与空质粒组相比，OPN-shRNA 质粒组兔扩张动脉的管腔面积明显增加，而斑块负荷明显减小。提示在动脉粥样硬化兔模型局部血管利用球囊导管能成功地转导OPN-shRNA质粒，被扩张血管的再狭窄程度减轻，血栓负荷减轻。这对于预防模型兔血管成形后再狭窄的发生具有十分重要的意义。
배경：혈관성형후재협착엄중한제료경피관상동맥개입치료적응용화원기료효。평활기세포적표형전변，증식시혈관성형후재협착적중요궤제。 　　목적：탐토이용구낭재체전도골교단백단발협상RNA，통과억제실험성동맥죽양경화모형토손상혈관부위골교단백적표체，예방혈관성형후재협착。 　　방법：구건동맥죽양경화모형토20지，수궤등분성공질립조화OPN-shRNA질립조，분별이용구낭재복주동맥도입OPN-shRNA질립화공재체。 　　결과여결론：2조토구낭확장후혈관평활기층출현특이성록색형광，차수전염후시간적연장형광강도축점강저，여공질립조상비，OPN-shRNA 질립조토확장동맥적관강면적명현증가，이반괴부하명현감소。제시재동맥죽양경화토모형국부혈관이용구낭도관능성공지전도OPN-shRNA질립，피확장혈관적재협착정도감경，혈전부하감경。저대우예방모형토혈관성형후재협착적발생구유십분중요적의의。
BACKGROUND:Restenosis after angioplasty severely limited the application and long-period therapeutic effects of percutaneous coronary intervention. Changes in smooth muscle cel phenotype and their proliferation are important mechanisms of restenosis after angioplasty. OBJECTIVE:To use bal oon in vivo transduction of osteopontin short hairpin RNA (OPN-shRNA), to inhibit osteopontin expression at the injured blood vessels of a rabbit model of experimental atherosclerosis, and to prevent restenosis after angioplasty. METHODS:A total of 20 rabbit models of atherosclerosis were established and randomly equal y assigned to empty plasmid group and OPN-shRNA plasmid group. The plasmid recombinant OPN-shRNA and empty plasmid were transferred to the ventral aorta by bal oon. RESULTS AND CONCLUSION:After bal oon dilatation, specific green fluorescence was detected in the layer of vascular smooth muscle in the two groups. Moreover, with prolonged time of transfection, fluorescence intensity gradual y decreased. Compared with the empty plasmid group, the expanded artery lumen area obviously increased in the OPN-shRNA plasmid group, and plaque burden evidently reduced. Results indicated that bal oon catheter used in regional blood vessels in rabbit models of atherosclerosis could successful y transduce OPN-shRNA plasmid. The restenosis of the expanded blood vessels lessened, and thrombus burden relieved. It is of great importance to prevent the occurrence of restenosis after angioplasty in rabbit models.