CAJ | 학술논문

目的：研究尿多酸肽（CDA-2）作用于转移性肺肿瘤小鼠的机制。方法通过注射鼠Lewis 肺癌细胞到C57BL/6小鼠体内建立小鼠转移性肺癌模型。采用HE染色对小鼠肺部肿瘤数、肿瘤大小进行评价。利用Kaplan-Meier （K-M）生存率曲线分析小鼠生存情况。免疫组化方法检测小鼠肿瘤增殖抗原 Ki-67的表达；TUNEL凋亡免疫组化染色观察肿瘤细胞的凋亡。结果经CDA-2治疗后，小鼠的肺肿瘤数和最大肿瘤尺寸均显著低于 PBS组肿瘤小鼠，并且CDA-2呈剂量依赖性抑制肿瘤的生长（均P＜0.05）。K-M结果显示，使用PBS组肿瘤小鼠的生存时间显著低于CDA-2治疗组，且随着CDA-2剂量增加肿瘤小鼠生存时间不断延长。免疫组化染色结果显示，肺肿瘤小鼠经CDA-2治疗后，Ki-67阳性肿瘤细胞数显著低于PBS治疗的肿瘤小鼠（P＜0.05）；而肿瘤凋亡细胞数却明显高于 PBS组（P＜0.001）。结论 CDA-2可抑制小鼠肺癌的生长；延长肺癌小鼠的生存期；抑制肿瘤细胞增殖及诱导肿瘤细胞凋亡。
목적：연구뇨다산태（CDA-2）작용우전이성폐종류소서적궤제。방법통과주사서Lewis 폐암세포도C57BL/6소서체내건립소서전이성폐암모형。채용HE염색대소서폐부종류수、종류대소진행평개。이용Kaplan-Meier （K-M）생존솔곡선분석소서생존정황。면역조화방법검측소서종류증식항원 Ki-67적표체；TUNEL조망면역조화염색관찰종류세포적조망。결과경CDA-2치료후，소서적폐종류수화최대종류척촌균현저저우 PBS조종류소서，병차CDA-2정제량의뢰성억제종류적생장（균P＜0.05）。K-M결과현시，사용PBS조종류소서적생존시간현저저우CDA-2치료조，차수착CDA-2제량증가종류소서생존시간불단연장。면역조화염색결과현시，폐종류소서경CDA-2치료후，Ki-67양성종류세포수현저저우PBS치료적종류소서（P＜0.05）；이종류조망세포수각명현고우 PBS조（P＜0.001）。결론 CDA-2가억제소서폐암적생장；연장폐암소서적생존기；억제종류세포증식급유도종류세포조망。
Objective The aim of the study was to investigate the ef ect of CDA-2 on lung tumor in mice tumor models. Methods C57BL/6 mice were used to establish a lung cancer model by intravenous injection of LLC cells. Tumorigenesis was evaluated by tumor multiplicity and maximal tumor sizes. Kaplan Meier (K-M) survival curve was used to analyze survival rate of mice. The expression of Ki-67 in the tumor tissue was measured by immunohistochemical analysis. The apoptosis of tumor cells was analyzed by TUNEL staining. Results Lung tumor mice treated with CDA-2 significantly decreased lung tumor multiplicity and maximal tumor sizes compared to PBS group and depended on CDA-2 does inhibition(P<0.05). K-M survival analysis showed CDA-2-treated tumor mice significant prolonged the life time compared to PBS-treated mice. The numbers of Ki-67-positive tumor cells were significant lower in CDA-2-treated tumor mice than PBS-treated mice (P<0.05); In contrast, lung tumor cells showed significant rate of apoptosis and death when CDA-2 treatment (P<0.001). Conclusions CDA-2 inhibited lung tumor growth in mice;prolonging the life span of lung tumor mice;inhibiting tumor cells proliferation and inducing tumor cells apoptosis.