CAJ | 학술논문

背景：各种原因导致的较大骨缺损一直是临床修复的难题，建立标准的临界骨缺损动物模型，对评价各种成骨材料及技术的成骨效果具有重要的意义。 　　目的：建立兔颅顶骨临界骨缺损模型，确定颅顶骨有限的时间内临界骨缺损值。 　　方法：选取10只新西兰大白兔，以颅中缝为界，随机在颅顶骨上分别制备4个直径为4，5，6，7 mm的圆形全层骨缺损，建立兔颅顶骨临界骨缺损模型。通过大体解剖观察，X射线影像观察，锥形束CT观察并测定缺损区新生骨的骨密度，组织学检查，以评价各缺损区骨愈合情况。 　　结果与结论：建模后12周，4 mm组骨自愈能力显著，部分出现完整的骨桥连接。骨密度定量分析和骨小梁面积检测结果显示，4 mm组缺损区骨密度灰度值和相同放大倍数且同一视野下分析骨小梁面积值均明显高于其他3组(P <0.001)。5，6，7 mm组只在缺损区周缘出现少量成骨，缺损中央区主要被纤维结缔组织所充填。结果证实，实验成功建立了兔颅顶骨临界骨缺损模型，在缺损12周的观察期内，直径≥5 mm的骨缺损不能自行愈合，符合临界骨缺损的标准，可作为兔颅顶临界骨缺损参考值。
배경：각충원인도치적교대골결손일직시림상수복적난제，건립표준적림계골결손동물모형，대평개각충성골재료급기술적성골효과구유중요적의의。 　　목적：건립토로정골림계골결손모형，학정로정골유한적시간내림계골결손치。 　　방법：선취10지신서란대백토，이로중봉위계，수궤재로정골상분별제비4개직경위4，5，6，7 mm적원형전층골결손，건립토로정골림계골결손모형。통과대체해부관찰，X사선영상관찰，추형속CT관찰병측정결손구신생골적골밀도，조직학검사，이평개각결손구골유합정황。 　　결과여결론：건모후12주，4 mm조골자유능력현저，부분출현완정적골교련접。골밀도정량분석화골소량면적검측결과현시，4 mm조결손구골밀도회도치화상동방대배수차동일시야하분석골소량면적치균명현고우기타3조(P <0.001)。5，6，7 mm조지재결손구주연출현소량성골，결손중앙구주요피섬유결체조직소충전。결과증실，실험성공건립료토로정골림계골결손모형，재결손12주적관찰기내，직경≥5 mm적골결손불능자행유합，부합림계골결손적표준，가작위토로정림계골결손삼고치。
BACKGROUND:Large bone defect caused by various reasons has been a difficult problem in clinical practice. To establish a standard experimental animal model of critical bone defects has vital significance for evaluating the efficacy of bone osteogenesis using various materials and techniques. OBJECTIVE:To establish the rabbit model of parietal critical bone defects and to determine the diameter of the critical defects of parietal bone in limited time. METHODS:10 New Zealand white rabbits were selected. The skul seam was treated as the boundary. Four ful-thickness round defects of bone in the parietal bone were made, with diameters of 4, 5, 6 and 7 mm, so as to establish rabbit models of parietal critical bone defects. Gross anatomical observation, X-ray and cone beam CT were used to determine the bone density in the new bone defect area. The healing of bone defects was evaluated by histological examination. RESULTS AND CONCLUSION:After 12 weeks, the 4 mm group showed high bone healing capacity significantly, and part of the bone bridge had been connected completely. Quantitative analysis of bone mineral density revealed that gray value at defect site and trabecular bone area at the same magnification and the same vision in the 4 mm group were significantly higher than the other three groups (P<0.001). Only a smal amount of new bone in the periphery of bone defects appeared in the 5, 6 and 7 mm groups. The center of defect site was mainly fil ed by fibrous connective tissue. The results confirmed that this study successful y established rabbit models of parietal critical bone defects. During the 12 weeks of observation, bone defects with a diameter of ≥ 5 mm could not be self-healed, which was conformed to the criteria of critical defects of bone, and could be used as a reference value for critical parietal bone defects of a rabbit.