CAJ | 학술논문

目的：通过对不同培养时间及秋水仙素处理条件、滴片高度等进行比较，建立本室较成熟的外周血淋巴细胞染色体制备技术。方法随机选择5例待测外周血淋巴细胞染色体患者作为实验对象，采集肝素抗凝静脉全血2ml，各接种4瓶，其中培养时间分别为62h、70h，秋水仙素处理条件为1h（终浓度0.2滋g/ml）、3h（终浓度0.08滋g/ml），滴片高度为2cm、10cm和30cm。计算不同处理条件下的细胞分裂指数，并对结果进行统计分析。结果培养62h和70h均可获得较多分裂相，两者比较差异无统计学意义（P>0.05）；秋水仙素处理3h（终浓度0.08滋g/ml）的分裂相比处理1h（终浓度0.2滋g/ml）明显增多（P<0.05）；3种滴片高度均可获得染色体分散良好的分裂相。结论外周血淋巴细胞经过62~70h培养及0.08滋g/ml秋水仙素处理3h后，可获得良好的染色体分裂相，滴片高度对染色体的分散无影响。
목적：통과대불동배양시간급추수선소처리조건、적편고도등진행비교，건립본실교성숙적외주혈림파세포염색체제비기술。방법수궤선택5례대측외주혈림파세포염색체환자작위실험대상，채집간소항응정맥전혈2ml，각접충4병，기중배양시간분별위62h、70h，추수선소처리조건위1h（종농도0.2자g/ml）、3h（종농도0.08자g/ml），적편고도위2cm、10cm화30cm。계산불동처리조건하적세포분렬지수，병대결과진행통계분석。결과배양62h화70h균가획득교다분렬상，량자비교차이무통계학의의（P>0.05）；추수선소처리3h（종농도0.08자g/ml）적분렬상비처리1h（종농도0.2자g/ml）명현증다（P<0.05）；3충적편고도균가획득염색체분산량호적분렬상。결론외주혈림파세포경과62~70h배양급0.08자g/ml추수선소처리3h후，가획득량호적염색체분렬상，적편고도대염색체적분산무영향。
Objective Through comparing the dif erent culture time, colchicine treatment condition and drop height to establish a mature peripheral blood lymphocyte chromosome preparation method. Methods 5 cases were randomly recruited as the subjects and 2ml peripheral blood of everybody was col ected which was inoculating in four bot les. The incubation time was 62h and 70h,the colchicine condition is 0.2μg/ml final concentration treating for 1 hour and 0.08μg/ml final concentration treating for 3 hours ,the drop heights are 2cm,10cm and 30cm. Mitotic index under dif erent conditions was calculated for statistical y analyzing. Results Both the two culture time can harvest a lot of cleavage phases, there was no significant dif erence (P>0.05); when comparing with the 1 hour treating condition of colchicine with 0.2μg/ml final concentration, the cleavage phases of colchicine with 0.08?g/ml final concentration treating increased significantly (P<0.05);3 drop heights can gain good metaphase speading. Conclusion: When cultured for 62-70 hours and treated with colchicine of 0.08μg/ml final concentration for 3 hours, the good metaphase chromosome of peripheral blood lymphocytes can be aquired successful y.There is no influence of drop height on chromosome speading.