CAJ | 학술논문

背景：目前关于维甲酸对干细胞增殖作用的研究较少见，对睾酮的研究主要集中于其抑制细胞衰老的作用方面。目的：探索维甲酸和睾酮单独和联合诱导对脂肪源干细胞细胞周期的影响。方法：分离培养2月龄SD雌性大鼠脂肪源干细胞，传至第3代进行成脂、成骨诱导和表面标记鉴定。将其分为6组：①对照组。②10-5 mol/L 维甲酸组。③10-6 mol/L 维甲酸组。④10-5 mol/L维甲酸+睾酮组。⑤10-6 mol/L维甲酸+睾酮组。⑥睾酮组。对照组应用DMEM+体积分数10%胎牛血清培养液，其余各组均在对照组的基础上加相应剂量维甲酸或睾酮或二者联合诱导剂。各组培养36 h后行流式细胞仪检测各细胞分期的变化。结果与结论：与对照组相比，10-5 mol/L 维甲酸组和10-6 mol/L 维甲酸组G 1期细胞数比例明显升高，S期细胞数比例明显降低。与对照组相比，睾酮组G 1期细胞数比例明显下降，S期细胞数比例为明显上升。10-5 mol/L维甲酸+睾酮组与10-5 mol/L 维甲酸组比较，10-6 mol/L维甲酸+睾酮组与10-6 mol/L 维甲酸组比较，G 1期细胞数比例有所下降，S期细胞数比例为明显上升。结果表明，维甲酸可将脂肪源干细胞周期阻滞于G 1期，延缓细胞周期由G1期向S期的进程；睾酮可加速脂肪源干细胞周期由G1期向S期的进程；联合诱导可加速脂肪源干细胞的细胞周期由G1期向S期的进程。
배경：목전관우유갑산대간세포증식작용적연구교소견，대고동적연구주요집중우기억제세포쇠로적작용방면。목적：탐색유갑산화고동단독화연합유도대지방원간세포세포주기적영향。방법：분리배양2월령SD자성대서지방원간세포，전지제3대진행성지、성골유도화표면표기감정。장기분위6조：①대조조。②10-5 mol/L 유갑산조。③10-6 mol/L 유갑산조。④10-5 mol/L유갑산+고동조。⑤10-6 mol/L유갑산+고동조。⑥고동조。대조조응용DMEM+체적분수10%태우혈청배양액，기여각조균재대조조적기출상가상응제량유갑산혹고동혹이자연합유도제。각조배양36 h후행류식세포의검측각세포분기적변화。결과여결론：여대조조상비，10-5 mol/L 유갑산조화10-6 mol/L 유갑산조G 1기세포수비례명현승고，S기세포수비례명현강저。여대조조상비，고동조G 1기세포수비례명현하강，S기세포수비례위명현상승。10-5 mol/L유갑산+고동조여10-5 mol/L 유갑산조비교，10-6 mol/L유갑산+고동조여10-6 mol/L 유갑산조비교，G 1기세포수비례유소하강，S기세포수비례위명현상승。결과표명，유갑산가장지방원간세포주기조체우G 1기，연완세포주기유G1기향S기적진정；고동가가속지방원간세포주기유G1기향S기적진정；연합유도가가속지방원간세포적세포주기유G1기향S기적진정。
BACKGROUND:The researches about the effect of retinoic acid on the proliferation of adipose-derived stem cells are rare, and the researches on the testosterone are mainly on the inhibition of cellaging. OBJECTIVE: To study the effects of retinoic acid and testosterone or combination on the cellcycle of adipose derived stem cells. METHODS:Adipose derived stem cells were isolated from adult female Sprague Dawley rats with 2 months age and cultured in vitro til passage 3 adipose derived stem cells, and then the 3rd passage adipose-derived stem cells were performed with adipogenic induction, osteogenic induction and surface marker identification. The cells were divided into six groups:(1) Control group;(2) 10-5 mol/L retinoic acid group;(3) Retinoic acid group;(4) 10-5 mol/L retinoic acid+testosterone group;(5) 10-6 mol/L retinoic acid+testosterone group;(6) Testosterone group. The adipose-derived stem cells in the control group were cultured with Dulbecco’s modified Eagle’s medium+10%fetal bovine serum culture medium, and the adipose-derived stem cells in the other five groups were induced with corresponding dose of retinoic acid and testosterone on the basis of control group. After cultured for 36 hours, the flow cytometry was used to detect the changes of cellcycle. RESULTS AND CONCLUSION:Compared with the control group, cellproportions in phase G 1 of 10-5 mol/L retinoic acid group and 10-6 mol/L retinoic acid group were increased significantly, and the cellproportions in phase S were decreased. Compared with control group, the cellproportion in phase G 1 of testosterone group was significantly reduced, and the cellproportion in phase S was increased. Compared with 10-5 mol/L retinoic acid group and 10-6 mol/L retinoic acid group, cellproportions in phase G 1 of 10-5 mol/L retinoic acid+testosterone group and 10-6 mol/L retinoic acid+testosterone group were reduced significantly and the cellproportions in phase S were increased. Retinoic acid can inhibit the cellcycle of adipose-derived stem cells in phase G 1 , and delay the process of the cellcycle from phase G1 to phase S;while testosterone can promote the cellcycle of adipose-derived stem cells from phase G1 to phase S;the combination induction of retinoic acid and testosterone can accelerate the process of the cellcycle of adipose-derived stem cells from phase G 1 to phase S.