CAJ | 학술논문

背景：肌肉注射人脐带间充质干细胞是否会增加正常心肌细胞的再生能力，对正常心肌是否会产生不良影响，尚不明确。目的：观察肌肉注射人脐带间充质干细胞对健康Wistar大鼠心肌组织中ki-67、phh-3及cTnT表达的影响。方法：将60只健康雄性Wistar大鼠随机分为正常组，培养液组，上清液组，脐带间充质干细胞低、中、高剂量组，每组分别给予大鼠四肢肌肉注射不同液体，分别为：PBS、DMEM培养液、培养人脐带间充质干细胞的上清液、0.25×105人脐带间充质干细胞、1.0×105人脐带间充质干细胞、4.0×105人脐带间充质干细胞，间隔4周共注射2次，第8周处死大鼠，取心肌组织固定、包埋及切片，应用免疫组织化学方法检测大鼠心肌组织中ki-67、phh-3及cTnT的表达。结果与结论：正常组中ki-67在心肌组织细胞胞核呈阴性表达，phh3在心肌组织细胞胞核呈弱阳性表达，cTnT在心肌细胞胞浆中呈强阳性表达；与正常组相比，培养液组、上清液组及脐带间充质干细胞低、中、高剂量组间ki-67、phh-3及cTnT的表达差异无显著性意义(F=1.076、0.167及0.300；P>0.05)。提示经肌肉注射人脐带间充质干细胞或其培养上清液对健康Wistar大鼠心肌组织ki-67、phh-3及cTnT的表达无影响。
배경：기육주사인제대간충질간세포시부회증가정상심기세포적재생능력，대정상심기시부회산생불량영향，상불명학。목적：관찰기육주사인제대간충질간세포대건강Wistar대서심기조직중ki-67、phh-3급cTnT표체적영향。방법：장60지건강웅성Wistar대서수궤분위정상조，배양액조，상청액조，제대간충질간세포저、중、고제량조，매조분별급여대서사지기육주사불동액체，분별위：PBS、DMEM배양액、배양인제대간충질간세포적상청액、0.25×105인제대간충질간세포、1.0×105인제대간충질간세포、4.0×105인제대간충질간세포，간격4주공주사2차，제8주처사대서，취심기조직고정、포매급절편，응용면역조직화학방법검측대서심기조직중ki-67、phh-3급cTnT적표체。결과여결론：정상조중ki-67재심기조직세포포핵정음성표체，phh3재심기조직세포포핵정약양성표체，cTnT재심기세포포장중정강양성표체；여정상조상비，배양액조、상청액조급제대간충질간세포저、중、고제량조간ki-67、phh-3급cTnT적표체차이무현저성의의(F=1.076、0.167급0.300；P>0.05)。제시경기육주사인제대간충질간세포혹기배양상청액대건강Wistar대서심기조직ki-67、phh-3급cTnT적표체무영향。
BACKGROUND:It is unclear whether intramuscular injection of human umbilical cord mesenchymal stem cells wil increase regeneration of normal myocardial cells and play adverse effects on normal myocardium. OBJECTIVE:To observe the effects of intramuscular injection of human umbilical cord mesenchymal stem cells on expression of the myocardial ki-67, phh3 and cTnT in normal Wistar rats. METHODS:A total of 60 male Wistar rats were divided into six groups randomly:normal group, solution group, supernatant group, low concentration group, moderate concentration group, and high concentration group. These groups were intramuscularly injected different liquid, respectively, as fol ows:PBS, DMEM, the supernatant of human umbilical cord mesenchymal stem cells, human umbilical cord mesenchymal stem cells with amount of 0.25×105, human umbilical cord mesenchymal stem cells with amount of 1.0×105, human umbilical cord mesenchymal stem cells with amount of 4.0×105 . After 4 weeks, each rats received the second same intramuscular injections of human umbilical cord mesenchymal stem cells. Four weeks after the second injection, al rates were kil ed to obtain myocardial tissues which were fixed, embedded and sectioned. Final y, the ki-67, phh3 and cTnT expressions of the myocardium were detected by immunohistochemical technique. RESULTS AND CONCLUSION:In the normal group, negative expression of the ki-67 was observed in the caryon of myocardial cells, weak positive expression of the phh3 was observed in the caryon of myocardial cells, and positive expression of the cTnT was observed in the caryon of myocardial cells. Compared with the normal group, the expression of the ki-67, phh3 and cTnT in the myocardium had no significant difference among the other groups (F=1.076, 0.167, 0.300;P>0.05). Human umbilical cord mesenchymal stem cells or the supernatant of human umbilical cord mesenchymal stem cells via intramuscular injection have no effects on the expression of the ki-67, phh3 and cTnT in normal Wistar rats.