CAJ | 학술논문

背景：人工肝组织有可能为治疗急性肝衰竭提供一种新的选择，但人工肝组织的构建存在着种子细胞、支架、培养模式及对人工肝组织营养获取调控等各方面的难题。 　　目的：体外构建3种人工肝组织，筛选最佳人工肝组织以供移植用。 　　方法：纯化、扩增并诱导分化骨髓间充质干细胞为肝样细胞，在 Transwel 培养体系中分别与去细胞羊膜、DACRON PATCH心血管外科补片、生物外科补片3种支架结合构建3种人工肝组织，培养3 d后进行形态学和功能学检测。 　　结果与结论：密度梯度离心结合贴壁法可获得较高纯度的大鼠骨髓间充质干细胞，并在体外转分化为肝样细胞。在构建的3种人工肝组织中，肝样细胞与生物外科补片支架材料可获得最大程度结合，并具有更强的尿素合成能力和白蛋白分泌功能，为治疗急性肝衰竭提供了实验基础。
배경：인공간조직유가능위치료급성간쇠갈제공일충신적선택，단인공간조직적구건존재착충자세포、지가、배양모식급대인공간조직영양획취조공등각방면적난제。 　　목적：체외구건3충인공간조직，사선최가인공간조직이공이식용。 　　방법：순화、확증병유도분화골수간충질간세포위간양세포，재 Transwel 배양체계중분별여거세포양막、DACRON PATCH심혈관외과보편、생물외과보편3충지가결합구건3충인공간조직，배양3 d후진행형태학화공능학검측。 　　결과여결론：밀도제도리심결합첩벽법가획득교고순도적대서골수간충질간세포，병재체외전분화위간양세포。재구건적3충인공간조직중，간양세포여생물외과보편지가재료가획득최대정도결합，병구유경강적뇨소합성능력화백단백분비공능，위치료급성간쇠갈제공료실험기출。
BACKGROUND:Engineered hepatic tissue is considered a promising strategy for healing acute liver failure. But, there are series of hindrances in the construction of engineered hepatic tissues, including acquisition of vital hepatocytes, choice of scaffolds and culture system, and nutrition supply. OBJECTIVE:To construct three kinds of engineered hepatic tissues in hope to screen the optimal one for transplantation in acute liver failure. METHODS:After purification, amplification, bone marrow mesenchymal stem cells were induced to differentiate into hepatocyte-like cells which were co-cultured with acellular amniotic membrane, DACRON PATCH cardiovascular surgical patch, biological surgical patch, respectively to construct three kinds of engineered hepatic tissues. After 3 days of culture, morphological and functional detections were performed. RESULTS AND CONCLUSION:Rat bone marrow mesenchymal stem cells with higher purity were successful y harvested by using density gradient centrifugation and adherent methods, and then the cells were differentiated into hepatocyte-like cells. In the three kinds of engineered hepatic tissues, hepatocyte-like cells were found to be combined with the biological surgical patch to the maximum extent, and their combination exhibited stronger ability of urea synthesis and albumin secretion, which provides experimental basis for treatment of acute liver failure.