CAJ | 학술논문

移植肾急性抗体介导排斥反应中半乳糖凝集素7的表达
이식신급성항체개도배척반응중반유당응집소7적표체
Expression of galectin-7 in acute antibody-mediated rejection after renal transplantation

万方数据

中国组织工程研究中國組織工程研究 중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年 42期 6884-6888 ,共5页

韩永%郭晖%蔡明%钱叶勇%李州利%周文强%王强%许晓光%黄海燕%肖漓%王新颖%石炳毅

한영%곽휘%채명%전협용%리주리%주문강%왕강%허효광%황해연%초리%왕신영%석병의

组织构建%组织工程%半乳糖凝集素7%肾移植%急性抗体介导性排斥反应%免疫组织化学%免疫印记%国家自然科学基金組織構建%組織工程%半乳糖凝集素7%腎移植%急性抗體介導性排斥反應%免疫組織化學%免疫印記%國傢自然科學基金
조직구건%조직공정%반유당응집소7%신이식%급성항체개도성배척반응%면역조직화학%면역인기%국가자연과학기금
kidney transplantation%galectins%graft rejection

背景：半乳糖凝集素家族蛋白在移植免疫调节中的作用已被提出，目前尚未有半乳糖凝集素7检测对于肾移植后围手术期移植肾功能不全的辅助诊断，对肾移植受者而言，监测半乳糖凝集素7可能及早发现移植后肾功能异常，为临床治疗争取时间。 　　目的：检测半乳糖凝集素7在移植肾急性抗体介导性排斥反应中的表达。 　　方法：选取同种异体肾移植后行移植肾穿刺活检诊断为急性抗体介导性排斥反应的患者27例，同时选取同期移植后肾病理穿刺经诊断为正常的患者10例做对照。应用免疫组织化学染色法检测半乳糖凝集素7在组织中的表达情况、应用Western Blot法检测各组血清半乳糖凝集素7蛋白表达水平差异。 　　结果与结论：免疫组织化学正常组光镜下半乳糖凝集素7表达于近曲小管上皮细胞表面微绒毛，肾小球、远曲小管、集合管和静脉未见其表达；急性抗体介导的体液性排斥反应组光镜下肾小动脉内膜水肿，管壁纤维素样坏死，肾小球肾小管淋巴细胞和单核细胞浸润，半乳糖凝集素7仅见于近曲小管上皮细胞表面微绒毛，同时动脉平滑肌也有表达，阳性度较高。急性抗体介导的体液性排斥反应组半乳糖凝集素7阳性细胞计数显著高于正常对照组(P<0.01)。Western Blot检测结果，急性抗体介导的体液性排斥反应组血清半乳糖凝集素7的表达高于正常对照组(P<0.05)。结果说明，移植肾穿刺病理活检安全可靠，对患者及移植肾无不良影响，半乳糖凝集素7检测对于肾移植后围手术期移植肾功能不全的辅助诊断具有重要的指导意义。
배경：반유당응집소가족단백재이식면역조절중적작용이피제출，목전상미유반유당응집소7검측대우신이식후위수술기이식신공능불전적보조진단，대신이식수자이언，감측반유당응집소7가능급조발현이식후신공능이상，위림상치료쟁취시간。 　　목적：검측반유당응집소7재이식신급성항체개도성배척반응중적표체。 　　방법：선취동충이체신이식후행이식신천자활검진단위급성항체개도성배척반응적환자27례，동시선취동기이식후신병리천자경진단위정상적환자10례주대조。응용면역조직화학염색법검측반유당응집소7재조직중적표체정황、응용Western Blot법검측각조혈청반유당응집소7단백표체수평차이。 　　결과여결론：면역조직화학정상조광경하반유당응집소7표체우근곡소관상피세포표면미융모，신소구、원곡소관、집합관화정맥미견기표체；급성항체개도적체액성배척반응조광경하신소동맥내막수종，관벽섬유소양배사，신소구신소관림파세포화단핵세포침윤，반유당응집소7부견우근곡소관상피세포표면미융모，동시동맥평활기야유표체，양성도교고。급성항체개도적체액성배척반응조반유당응집소7양성세포계수현저고우정상대조조(P<0.01)。Western Blot검측결과，급성항체개도적체액성배척반응조혈청반유당응집소7적표체고우정상대조조(P<0.05)。결과설명，이식신천자병리활검안전가고，대환자급이식신무불량영향，반유당응집소7검측대우신이식후위수술기이식신공능불전적보조진단구유중요적지도의의。
BACKGROUND:The role of galactose lectin family proteins in transplantation immunity has been proposed, but there is currently no galectin-7 detection for auxiliary diagnosis of renal dysfunction in the perioperative period after renal transplantation. For renal transplant recipients, monitoring of galectin-7 may contribute to early diagnosis of renal dysfunction after renal transplantation, and buy time for clinical treatment. OBJECTIVE:To detect the expression of galactose-7 in acute antibody mediated rejection after renal transplantation. METHODS:Twenty-seven patients who were diagnosed as having acute antibody mediated rejection after renal transplantation by renal biopsy were enrol ed, and another 10 patients without acute antibody mediated rejection after renal transplantation were selected as controls. Immunohistochemical staining and western blot assay were used to detect expression of galectin-7 in tissue and serum, respectively. RESULTS AND CONCLUSION:Results of immunohistochemistry staining showed that under light microscope, in the control group, galectin-7 distributed in the surface microvil i of proximal tubule epithelial cells, but not in glomeruli, distal tubule, col ecting duct and vein;in the acute rejection group, renal arteriole intima edema, tube wal fibrinoid necrosis, infiltration of renal glomerulus and tubule cells and mononuclear cells were found and galectin-7 only expressed in the surface microvil i of proximal tubule epithelial cells as wel as in the arterial smooth muscle. The number of galectin-7 positive cells in the acute rejection group was significantly higher than that in the control group (P<0.1). Western blot assay results showed that the protein expression of serum galectin-7 in the acute rejection group was higher than that in the control group (P<0.05). These findings indicate that renal puncture for renal transplantation is safe and reliable, has no adverse effect on the patients and renal transplant. Galectin-7 detection has an important guiding significance for the auxiliary diagnosis of renal dysfunction during the perioperative period after renal transplantation.