CAJ | 학술논문

背景：视神经炎的病因多为自身免疫引起的炎性脱髓鞘，很多的视神经炎为多发性硬化的早期表现。在外周CD4+T细胞中，有5%-10%为调节性T细胞，其免疫抑制能力很强。自身免疫性脑脊髓炎所引起的视神经炎发病机制是否与辅助性T细胞亚群的有关呢？目的：分析实验性自身免疫性脑脊髓炎小鼠模型视神经炎发病机制与辅助性T细胞亚群的关系。方法：将小鼠腹腔注射百日咳菌液建立实验性自身免疫性脑脊髓炎模型，分别免疫11，15，19 d，并设腹腔注射生理盐水的佐剂组小鼠作对照。 　　结果与结论：酶联免疫吸附检测显示，与佐剂组相比，免疫后19 d组视神经白细胞介素4蛋白含量降低(P<0.05)；免疫后11，15 d组视神经白细胞介素17蛋白含量升高(P<0.05)；免疫后15，19 d组视神经干扰素γ蛋白含量升高(P <0.05)；免疫后11，15，19 d组视神经Foxp3蛋白含量显著降低(P <0.05)。实时PCR检测显示，与佐剂组相比，免疫后11，15，19 d组视神经中干扰素γ、Foxp3 mRNA表达降低(P<0.05)， RORt mRNA表达升高；免疫后15，19 d组视神经中白细胞介素4，白细胞介素17，T-beat mRNA表达升高(P<0.05)。免疫后19 d组GATA3 mRNA表达降低(P<0.05)。结果证实，实验性自身免疫性脑脊髓炎小鼠模型视神经炎的发生发展可能受到Foxp3和调节性T细胞表达减少的影响，免疫后早期白细胞介素17可能介导对炎症损伤，发病高峰期干扰素γ可能使炎症损伤程度加重。
배경：시신경염적병인다위자신면역인기적염성탈수초，흔다적시신경염위다발성경화적조기표현。재외주CD4+T세포중，유5%-10%위조절성T세포，기면역억제능력흔강。자신면역성뇌척수염소인기적시신경염발병궤제시부여보조성T세포아군적유관니？목적：분석실험성자신면역성뇌척수염소서모형시신경염발병궤제여보조성T세포아군적관계。방법：장소서복강주사백일해균액건립실험성자신면역성뇌척수염모형，분별면역11，15，19 d，병설복강주사생리염수적좌제조소서작대조。 　　결과여결론：매련면역흡부검측현시，여좌제조상비，면역후19 d조시신경백세포개소4단백함량강저(P<0.05)；면역후11，15 d조시신경백세포개소17단백함량승고(P<0.05)；면역후15，19 d조시신경간우소γ단백함량승고(P <0.05)；면역후11，15，19 d조시신경Foxp3단백함량현저강저(P <0.05)。실시PCR검측현시，여좌제조상비，면역후11，15，19 d조시신경중간우소γ、Foxp3 mRNA표체강저(P<0.05)， RORt mRNA표체승고；면역후15，19 d조시신경중백세포개소4，백세포개소17，T-beat mRNA표체승고(P<0.05)。면역후19 d조GATA3 mRNA표체강저(P<0.05)。결과증실，실험성자신면역성뇌척수염소서모형시신경염적발생발전가능수도Foxp3화조절성T세포표체감소적영향，면역후조기백세포개소17가능개도대염증손상，발병고봉기간우소γ가능사염증손상정도가중。
BACKGROUND:More and more evidence have shown that autoimmune-induced inflammatory demyelinating mostly leads to optic neuritis that is quite an early manifestation of multiple sclerosis, but whether the pathogenesis of optic neuritis in experimental autoimmune encephalomyelitis (EAE) mice is correlated with helper T cellsubsets has rarely been reported. OBJECTIVE:To analyze the correlation between pathogenesis of optic neuritis of mouse EAE model with helper T cellsubsets. METHODS:The mice were injected intraperitoneal y Bordetel a pertussis to establish EAE models. Then, the animal models were subjected to immunization for 11, 15, 19 days, respectively. Mice undergoing intraperitoneal injection of normal saline served as controls (adjuvant group). RESULTS AND CONCLUSION:Compared with the adjuvant group, the protein expression of interleukin 4 in the optic nerve decreased in the 19-day immunization group (P<0.05);the protein expression of interleukin 17 in the optic nerve increased in the 11-and 15-day immunization groups (P<0.05);the protein expression of interferonγin the optic nerve increased in the 15-and 19-day immunization groups (P<0.05);the protein expression of Foxp3 in the optic nerve decreased in the 11-, 15-and 19-day immunization groups (P<0.05). Real-time PCR results showed that compared with the adjuvant group, the mRNA expression of interferonγand Foxp3 in the optic nerve decreased (P<0.05), while mRNA expression of RORt increased in the 11-, 15-and 19-day immunization groups;the mRNA expression of interleukin 4, interleukin 17, T-beat increased in the 15-and 19-day immunization groups (P<0.05);the mRNA expression of GATA3 reduced in the 19-day immunization group (P<0.05). These results reveal that Foxp3 expression and helper T cellreduction have important influences on the development of optic neuritis in EAE mouse models, interleukin 17 may mediates inflammatory injury in the early stage, while interferon-γmakes inflammatory injury worse in the peak incidence of the disease.