中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2014年
42期
6758-6762
,共5页
雷霄%梁英魁%赵文锐%王升%郭峰%傅强
雷霄%樑英魁%趙文銳%王升%郭峰%傅彊
뢰소%량영괴%조문예%왕승%곽봉%부강
组织构建%组织工程%树突状细胞%凋亡%细胞表型%免疫功能%趋化因子
組織構建%組織工程%樹突狀細胞%凋亡%細胞錶型%免疫功能%趨化因子
조직구건%조직공정%수돌상세포%조망%세포표형%면역공능%추화인자
dendritic cells%chemotactic factors%immunity
背景:既往研究表明,不同剂量的电离辐射对树突状细胞的成熟、免疫功能等也可能有一定的影响。目的:分析不同浓度的18 F-FDG对体外培养的人外周血单个核细胞源性树突状细胞的成熟及免疫功能的影响。方法:人外周血单个核细胞源性树突状细胞诱导成熟后,分为5组,分别加入含PBS及92.5×104,185×104,370×104,740×104 Bq/mL浓度的18 F-FDG培养基,作用24 h后收集细胞,检测树突状细胞的凋亡率、细胞表型(CD1α、CD80、CD83、CD86、HLA-DR)表达、同种混合淋巴细胞反应及细胞培养上清液中的巨噬细胞炎性蛋白1α和单核细胞趋化因子1表达。结果与结论:740×104 Bq/mL浓度的18F-FDG可以引起树突状细胞的凋亡、CD86的表达下调,同种T细胞抗原提呈能力下降,单核细胞趋化因子1的分泌下降;其余浓度的18F-FDG对树突状细胞的成熟及免疫功能无明显影响。结果表明较低浓度的18F-FDG对树突状细胞的凋亡、成熟、抗原提呈、迁移能力等影响较小,可以在选择合适浓度的前提下作为一种树突状细胞细胞的体外标记物。
揹景:既往研究錶明,不同劑量的電離輻射對樹突狀細胞的成熟、免疫功能等也可能有一定的影響。目的:分析不同濃度的18 F-FDG對體外培養的人外週血單箇覈細胞源性樹突狀細胞的成熟及免疫功能的影響。方法:人外週血單箇覈細胞源性樹突狀細胞誘導成熟後,分為5組,分彆加入含PBS及92.5×104,185×104,370×104,740×104 Bq/mL濃度的18 F-FDG培養基,作用24 h後收集細胞,檢測樹突狀細胞的凋亡率、細胞錶型(CD1α、CD80、CD83、CD86、HLA-DR)錶達、同種混閤淋巴細胞反應及細胞培養上清液中的巨噬細胞炎性蛋白1α和單覈細胞趨化因子1錶達。結果與結論:740×104 Bq/mL濃度的18F-FDG可以引起樹突狀細胞的凋亡、CD86的錶達下調,同種T細胞抗原提呈能力下降,單覈細胞趨化因子1的分泌下降;其餘濃度的18F-FDG對樹突狀細胞的成熟及免疫功能無明顯影響。結果錶明較低濃度的18F-FDG對樹突狀細胞的凋亡、成熟、抗原提呈、遷移能力等影響較小,可以在選擇閤適濃度的前提下作為一種樹突狀細胞細胞的體外標記物。
배경:기왕연구표명,불동제량적전리복사대수돌상세포적성숙、면역공능등야가능유일정적영향。목적:분석불동농도적18 F-FDG대체외배양적인외주혈단개핵세포원성수돌상세포적성숙급면역공능적영향。방법:인외주혈단개핵세포원성수돌상세포유도성숙후,분위5조,분별가입함PBS급92.5×104,185×104,370×104,740×104 Bq/mL농도적18 F-FDG배양기,작용24 h후수집세포,검측수돌상세포적조망솔、세포표형(CD1α、CD80、CD83、CD86、HLA-DR)표체、동충혼합림파세포반응급세포배양상청액중적거서세포염성단백1α화단핵세포추화인자1표체。결과여결론:740×104 Bq/mL농도적18F-FDG가이인기수돌상세포적조망、CD86적표체하조,동충T세포항원제정능력하강,단핵세포추화인자1적분비하강;기여농도적18F-FDG대수돌상세포적성숙급면역공능무명현영향。결과표명교저농도적18F-FDG대수돌상세포적조망、성숙、항원제정、천이능력등영향교소,가이재선택합괄농도적전제하작위일충수돌상세포세포적체외표기물。
BACKGROUND:Previous studies have shown that different doses of ionizing radiation may have some impact on maturation and immune function of dendritic cells. OBJECTIVE:To evaluate the effect of 18 F-FDG with different concentrations on maturation and immune function of dendritic cells from human peripheral blood mononuclear cells in vitro. METHODS:Human peripheral blood mononuclear cells-derived mature dendritic cells were divided into five groups:PBS, 92.5×10 4, 185×104, 370×104, 740×104 Bq/mL 18 F-FDG were separately added into each group. Dendritic cells were col ected 24 hours later. Apoptosis rate, phenotypic expression (CD1α, CD80, CD83, CD86, HLA-DR), ability of mixed lymphocyte reaction and expression of macrophage inflammatory protein 1αand monocyte chemotactic factor-1 in cellculture supernatant were detected. RESULTS AND CONCLUSION:Apoptosis rate, phenotypic expression of CD86, ability of mixed lymphocyte reaction and expression of monocyte chemotactic factor-1 were down-regulated after culture in 740×10 4 Bq/mL 18F-FDG. However, 18F-FDG at other concentrations had no influence on maturation and immune function of dendritic cells. This study demonstrates that low-concentration 18F-FDG has little influence on apoptosis, maturation, antigen presentation, and migratory capacity of dendritic cells, and it may be selected at an appropriate concentration as a label for dendritic cells in vitro.