CAJ | 학술논문

目的：研究对慢性应激抑郁模型大鼠海马BDNF表达与认知功能损伤之间的关系。方法采用21d慢性不可预见的温和应激处理结合孤养制备抑郁大鼠模型。运用被动躲避实验评定大鼠的学习记忆能力，采用荧光定量PCR技术和免疫组织化学技术检测BDNF基因和蛋白的表达变化。结果抑郁组大鼠与正常对照组比较，体重、糖水消耗量、旷场试验运动均减少，被动规避缺陷，以上行为学改变差异均有统计学意义（P<0.05）。免疫组织化学和实时荧光定量PCR技术检测均显示：抑郁组大鼠海马各区BDNF蛋白和mRNA相对表达量均显著低于正常对照组，差异有统计学意义（P<0.01）。结论慢性应激抑郁大鼠海马区BDNF表达下降可使认知障碍加重。
목적：연구대만성응격억욱모형대서해마BDNF표체여인지공능손상지간적관계。방법채용21d만성불가예견적온화응격처리결합고양제비억욱대서모형。운용피동타피실험평정대서적학습기억능력，채용형광정량PCR기술화면역조직화학기술검측BDNF기인화단백적표체변화。결과억욱조대서여정상대조조비교，체중、당수소모량、광장시험운동균감소，피동규피결함，이상행위학개변차이균유통계학의의（P<0.05）。면역조직화학화실시형광정량PCR기술검측균현시：억욱조대서해마각구BDNF단백화mRNA상대표체량균현저저우정상대조조，차이유통계학의의（P<0.01）。결론만성응격억욱대서해마구BDNF표체하강가사인지장애가중。
Objectives To make clear of the relationship between brain-derived neurotrophic factor (BDNF) expression and cognitive impairment in depression rats. Methods Isolated-living condition and chronic unpredictable mild stress are applied successively to set up depression rat model . Learning and memory function is evaluated by passive avoidance test.Real Time-PCR and immunohistochemistry method are applied to detect the BDNF mRNA and BDNF protein. Results Compared with the normal control group,the depression group have significant changes (P<0.05):weight loss,the decrease of sucrose solution consumption,the drop of open-field behavior, the deficiency of passive avoid test. Compared with the normal control group,BDNF expression is significantly decreased in the hippocampus of the depression group (P<0.01). Conclusions The decreased expression of BDNF in the hippocampus of depression rats may make the cognitive impairment increase.