现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2013年
22期
142-144,148
,共4页
邵丹%吉骊%朱莉伟%蒋建新
邵丹%吉驪%硃莉偉%蔣建新
소단%길려%주리위%장건신
糠醛渣%啤酒糟%底物诱导%绿色木霉%纤维素酶
糠醛渣%啤酒糟%底物誘導%綠色木黴%纖維素酶
강철사%비주조%저물유도%록색목매%섬유소매
furfural residue%beer residue%substrate induction%Trichoderma viride%cellulase
以糠醛渣与啤酒糟为混合底物,对绿色木霉(CGMCC3.2941)液态发酵产纤维素酶的一些条件进行了考察和优化。结果表明:底物浓度为2%,糠醛渣:啤酒糟质量比9:1,在(29±1)益培养11 d时,绿色木霉(CGMCC3.2941)所产纤维素酶的滤纸酶活为0.189 FPU/mL,内切酶活为0.451 U/mL,外切酶活为0.392 U/mL。与单一底物糠醛渣相比,混合底物的滤纸酶活、内切酶活、外切酶活分别提高了45.0%、3.8%、38.3%。
以糠醛渣與啤酒糟為混閤底物,對綠色木黴(CGMCC3.2941)液態髮酵產纖維素酶的一些條件進行瞭攷察和優化。結果錶明:底物濃度為2%,糠醛渣:啤酒糟質量比9:1,在(29±1)益培養11 d時,綠色木黴(CGMCC3.2941)所產纖維素酶的濾紙酶活為0.189 FPU/mL,內切酶活為0.451 U/mL,外切酶活為0.392 U/mL。與單一底物糠醛渣相比,混閤底物的濾紙酶活、內切酶活、外切酶活分彆提高瞭45.0%、3.8%、38.3%。
이강철사여비주조위혼합저물,대록색목매(CGMCC3.2941)액태발효산섬유소매적일사조건진행료고찰화우화。결과표명:저물농도위2%,강철사:비주조질량비9:1,재(29±1)익배양11 d시,록색목매(CGMCC3.2941)소산섬유소매적려지매활위0.189 FPU/mL,내절매활위0.451 U/mL,외절매활위0.392 U/mL。여단일저물강철사상비,혼합저물적려지매활、내절매활、외절매활분별제고료45.0%、3.8%、38.3%。
The liquid state fermentation conditions were studied and optimized for cellulase production by Trichoderma viride(CGMCC3.2941)using beer residue and furfural residue as mixed substrates.The results showed that the optimal conditions were as followsthe substrate concentration 2%,the ratio of furfural residue to beer residue 9:1,fermentation time 11d and fermentation temperature(29±1)℃.Under these conditions,the filter paper activity,endoglucanase activity,and exoglucanase activity could be reached 0.189 FPU/mL,0.451 U/mL and 0.392 U/mL,respectively,which increased by 45.0%,3.8%and 38.3%contrasted to the substrate without beer residue.