CAJ | 학술논문

目的:探讨CD151对人脐带来源间充质干细胞(human umbilical cord mesenchymal stem cells,hUC-MSCs)生物学性状的影响.方法:使用siRNA干扰hUC-MSCs表面CD151,实验分为实验组(siRNA-CD151组)和对照组(siRNA-NC组)；流式细胞术检测干扰72 h后hUC-MSCs CD151及其它细胞表型,yon Kossa和油红O染色检测体外成骨、成脂诱导分化情况；流式细胞术分析细胞周期变化；real-time PCR检测hUC-MSCs CD151、肝细胞生长因子(hepatocyte growth factor,HGF)、转化生长因子β1(transforming growth factorβ1,TGF-β1)、环氧合酶2(cyclooxygenase 2,COX-2)和吲哚胺2,3-双加氧酶(indoleamine 2,3-dioxygenase,IDO)mRNA的表达量；ELISA检测hUC-MSCs HGF分泌量.结果:(1)流式细胞术检测发现干扰72 h hUC-MSCs实验组CD151(11.97±2.63 vs95.66±1.56;P ＜0.01)和CD105(93.66±0.21vs 83.37±0.71;P ＜0.05)较对照组显著降低；real-time PCR检测hUC-MSCs CD151 mRNA变化和流式结果一致.(2)给予siRNA-CD151后能够减缓细胞周期进展,表现为细胞G1期增多,S期减少.(3)实验组hUC-MSCs HGF和TGF-β1表达量低于对照组(P＜0.01),而COX-2表达量升高(P＜0.05),IDO无明显改变；ELISA检测显示实验组hUC-MSCs HGF分泌量较对照组下降(P＜0.01).结论:体外干扰CD151后hUC-MSCs仍保持干细胞表型,体外成脂诱导分化能力无明显变化,但成骨诱导分化能力、增殖能力和相关免疫调节因子表达发生改变.
목적:탐토CD151대인제대래원간충질간세포(human umbilical cord mesenchymal stem cells,hUC-MSCs)생물학성상적영향.방법:사용siRNA간우hUC-MSCs표면CD151,실험분위실험조(siRNA-CD151조)화대조조(siRNA-NC조)；류식세포술검측간우72 h후hUC-MSCs CD151급기타세포표형,yon Kossa화유홍O염색검측체외성골、성지유도분화정황；류식세포술분석세포주기변화；real-time PCR검측hUC-MSCs CD151、간세포생장인자(hepatocyte growth factor,HGF)、전화생장인자β1(transforming growth factorβ1,TGF-β1)、배양합매2(cyclooxygenase 2,COX-2)화신타알2,3-쌍가양매(indoleamine 2,3-dioxygenase,IDO)mRNA적표체량；ELISA검측hUC-MSCs HGF분비량.결과:(1)류식세포술검측발현간우72 h hUC-MSCs실험조CD151(11.97±2.63 vs95.66±1.56;P ＜0.01)화CD105(93.66±0.21vs 83.37±0.71;P ＜0.05)교대조조현저강저；real-time PCR검측hUC-MSCs CD151 mRNA변화화류식결과일치.(2)급여siRNA-CD151후능구감완세포주기진전,표현위세포G1기증다,S기감소.(3)실험조hUC-MSCs HGF화TGF-β1표체량저우대조조(P＜0.01),이COX-2표체량승고(P＜0.05),IDO무명현개변；ELISA검측현시실험조hUC-MSCs HGF분비량교대조조하강(P＜0.01).결론:체외간우CD151후hUC-MSCs잉보지간세포표형,체외성지유도분화능력무명현변화,단성골유도분화능력、증식능력화상관면역조절인자표체발생개변.