新疆医科大学学报
新疆醫科大學學報
신강의과대학학보
JOURNAL OF XINJIANG MEDICAL UNIVERSITY
2013年
12期
1740-1743
,共4页
郭炬亮%李新霞%热娜?卡斯木
郭炬亮%李新霞%熱娜?卡斯木
곽거량%리신하%열나?잡사목
糙枝金丝桃%金丝桃苷%正交试验%含量测定%提取方法
糙枝金絲桃%金絲桃苷%正交試驗%含量測定%提取方法
조지금사도%금사도감%정교시험%함량측정%제취방법
Hypericum scabrum L%Hyperoside%orthogonal design%concentration determination%extrac-tion method
目的:确立糙枝金丝桃药材的最佳提取方法。方法通过单因素考察,分析提取溶剂和方法、乙醇浓度、料液比、提取时间、提取次数等因素对金丝桃苷提取率的影响,并在此基础上进行正交试验,以金丝桃苷为对照品,用 HPLC 法测定含量,优化提取工艺。结果最佳提取工艺为:料液比1∶50,95℃水浴中回流30 min ,乙醇浓度70%,此条件下糙枝金丝桃中金丝桃苷含量为3.35 mg/g 。结论本方法简便可行,重复性好,可作为糙枝金丝桃药材的提取方法。
目的:確立糙枝金絲桃藥材的最佳提取方法。方法通過單因素攷察,分析提取溶劑和方法、乙醇濃度、料液比、提取時間、提取次數等因素對金絲桃苷提取率的影響,併在此基礎上進行正交試驗,以金絲桃苷為對照品,用 HPLC 法測定含量,優化提取工藝。結果最佳提取工藝為:料液比1∶50,95℃水浴中迴流30 min ,乙醇濃度70%,此條件下糙枝金絲桃中金絲桃苷含量為3.35 mg/g 。結論本方法簡便可行,重複性好,可作為糙枝金絲桃藥材的提取方法。
목적:학립조지금사도약재적최가제취방법。방법통과단인소고찰,분석제취용제화방법、을순농도、료액비、제취시간、제취차수등인소대금사도감제취솔적영향,병재차기출상진행정교시험,이금사도감위대조품,용 HPLC 법측정함량,우화제취공예。결과최가제취공예위:료액비1∶50,95℃수욕중회류30 min ,을순농도70%,차조건하조지금사도중금사도감함량위3.35 mg/g 。결론본방법간편가행,중복성호,가작위조지금사도약재적제취방법。
Objective To establish the best extraction method for Hypericum scabrum L .Methods Use Hyperoside as the reference substances ,HPLC method to determinate the concentration of Hyperoside . During the extracting process ,five main factors ,including extracting solvent and method ,concentration of extracting solution ,solid‐liquid ratio ,refluxing time and extracting times ,were studied by using single factor analysis method .And based on the single‐factor analysis ,the L9 (34 )orthogonal was designed and optimized the best conditions extracting the hyperoside from the Hypericum scabrum L .Results The op‐timal extracting condition :ethanol concentration 70% ,solid‐liquid ration 1∶50 ,95 ℃ water‐bath reflux 30min .According to the congdiction mentioned above ,the content of Hyperoside which were extracted from Hypericum scabrum L could reach 3 .35 mg /g .Conclusion The method established for Hypericum scabrum L is simple ,feasible and reproducible ,and it can be used as Hypericum scabrum L extracting method .