CAJ | 학술논문

目的对常见的甲真菌病分离菌株进行细胞外水解酶活性测定,以期发现可能与甲真菌病发病相关的毒力因子.方法诱导培养基为含甲碎片的培养基,受试菌株包括常见皮肤癣菌、非皮肤癣菌的标准菌株及临床分离自甲真菌病的红色毛癣菌.细胞外水解酶谱的测定体系采用法国生物-梅里埃公司的酶活性测试试剂盒API ZYM.结果与非皮肤癣菌相比,来自于含甲碎片培养基的皮肤癣菌的酯酶、酯脂肪酶、N-乙酰-β-氨基葡萄糖苷酶和α-甘露糖苷酶活性增加(P＜0.05).与非红色毛癣菌相比,来自于含甲碎片培养基的红色毛癣菌N-乙酰-β-氨基葡萄糖苷酶活性升高(P＜0.05).临床评分指数不同积分段甲真菌病的红色毛癣菌临床分离株除萘酚-AS-BI-磷酸水解酶外,其余酶的活性差异均无统计学意义(P＞0.05).结论在特定环境中,水解酶的活性与菌株的种类有关,并对甲真菌病的临床表现可能有一定影响.
목적 대상견적갑진균병분리균주진행세포외수해매활성측정,이기발현가능여갑진균병발병상관적독력인자.방법 유도배양기위함갑쇄편적배양기,수시균주포괄상견피부선균、비피부선균적표준균주급림상분리자갑진균병적홍색모선균.세포외수해매보적측정체계채용법국생물-매리애공사적매활성측시시제합API ZYM.결과 여비피부선균상비,래자우함갑쇄편배양기적피부선균적지매、지지방매、N-을선-β-안기포도당감매화α-감로당감매활성증가(P＜0.05).여비홍색모선균상비,래자우함갑쇄편배양기적홍색모선균N-을선-β-안기포도당감매활성승고(P＜0.05).림상평분지수불동적분단갑진균병적홍색모선균림상분리주제내분-AS-BI-린산수해매외,기여매적활성차이균무통계학의의(P＞0.05).결론 재특정배경중,수해매적활성여균주적충류유관,병대갑진균병적림상표현가능유일정영향.
Objective To determine the extracellular enzymatic activity of common pathogens for onychomycosis, in the hope of finding virulence factors associated with the pathogenesis of onychomycosis. Methods Strains tested in this study included standard strains of common dermatophyte and non-dermatophyte fungi as well as clinical isolates of Trichophyton rubrum from patients with onychomycosis. All the tested strains were cultured in medium containing nail fragments at 25 ℃ for 10 to 21 days followed by the determination of the nail fragment-containing medium, a significant increase was observed in the activities of esterase, esterase lipase, N-acetyl-β-glucosaminidase and α-mannosidase in dermatophytes compared with non-dermatophytes (all P ＜ 0.05 ), as well as in the activity of N-acetyl-β-glucosaminidase in Trichophyton rubrum compared with the other tested species of fungi (all P ＜ 0.05). No significant difference was noted in the activity of extracellular enzymes, except for that of naphthol-AS-BI-phosphohydrolase, between the isolates of Trichophyton rubrum from patients with different ranges of scoring clinical index for onychomycosis (SCIO). Conclusions In specific conditions, the extracellular enzymatic activity of fungi isolated from patients with onychomycosis is associated with fungal species, and may have a certain influence on the manifestations of anychomycosis.