国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
9期
1193-1195
,共3页
大川芎%即型凝胶%天麻素%阿魏酸%蒸发光散射检测器
大川芎%即型凝膠%天痳素%阿魏痠%蒸髮光散射檢測器
대천궁%즉형응효%천마소%아위산%증발광산사검측기
Dachuanxiong%In situ gel%Gastrodin%Ferulic acid%HPLC-DAD
目的 建立以高效液相色谱-蒸发光散射检测器测定大川芎即型凝胶中天麻素、阿魏酸含量的方法.方法 以C18为色谱柱,流动相为乙腈-0.1％磷酸溶液进行梯度洗脱,检测波长为天麻素270 nm、阿魏酸321 nm,流速为1 ml/min,柱温是25℃,进样量是20μl.结果 天麻素、阿魏酸分别在0.2605 ～ 1.563μg(r=0.9995)、0.107 ～ 0.642μg(r=0.9998)浓度范围内有良好的线性关系,平均回收率分别为100.1％、100.0％,RSD分别是0.1％、0.1％.结论 本方法简便、准确、专属性强,可用于该制剂的质量控制.
目的 建立以高效液相色譜-蒸髮光散射檢測器測定大川芎即型凝膠中天痳素、阿魏痠含量的方法.方法 以C18為色譜柱,流動相為乙腈-0.1％燐痠溶液進行梯度洗脫,檢測波長為天痳素270 nm、阿魏痠321 nm,流速為1 ml/min,柱溫是25℃,進樣量是20μl.結果 天痳素、阿魏痠分彆在0.2605 ～ 1.563μg(r=0.9995)、0.107 ～ 0.642μg(r=0.9998)濃度範圍內有良好的線性關繫,平均迴收率分彆為100.1％、100.0％,RSD分彆是0.1％、0.1％.結論 本方法簡便、準確、專屬性彊,可用于該製劑的質量控製.
목적 건립이고효액상색보-증발광산사검측기측정대천궁즉형응효중천마소、아위산함량적방법.방법 이C18위색보주,류동상위을정-0.1％린산용액진행제도세탈,검측파장위천마소270 nm、아위산321 nm,류속위1 ml/min,주온시25℃,진양량시20μl.결과 천마소、아위산분별재0.2605 ～ 1.563μg(r=0.9995)、0.107 ～ 0.642μg(r=0.9998)농도범위내유량호적선성관계,평균회수솔분별위100.1％、100.0％,RSD분별시0.1％、0.1％.결론 본방법간편、준학、전속성강,가용우해제제적질량공제.
Objective To establish a method for the determination of gastrodin and ferulic acid in Dachuanxiong in situ gel by HPLC-DAD.Methods HPLC-DAD was performed on a C18 column using acetonitrile-0.1％ phosphate acid solution as the mobile phase for gradient elution at a flow rate of 1ml/min.The detection wave length was at 270 nm for gastrodin and 321 nm for fenulic acid.The column temperature was 25 ℃ and the sample size was 20 μ l.Results The linear range of gastrodin and ferulic acid was 0.2605-1.563 μ g (r =0.9995),0.107-0.642μg (r =0.9998) respectively,the average recovery was 100.1％,100.0％ respectively,RSD were 0.1％ and 0.1％ respectively.Conclusion The method is simple,accurate,special and can be used for the quality control of Dachuanxiong in situ gel.
