CAJ | 학술논문

目的：比较我国大陆地区广州管圆线虫线粒体基因组的多态性。方法在种群遗传学研究基础上，选取7条雌虫进行线粒体基因组的测定。根据广州管圆线虫线粒体基因组已知序列（GQ398121）设计12对引物，进行PCR扩增，并对目的片段进行测序和拼接。利用多种生物学软件对测定的线粒体基因组进行基因定位、结构图绘制、核苷酸及变异位点分析、进化关系分析。结果获得5个不同遗传类型的线粒体基因组。这些基因组的大小相似、结构相同，即13491～13502对碱基，含12个蛋白编码基因，2个核糖体基因，22个tRNA基因，2个较大的非编码区。上述基因紧密地排列在同一条DNA链上，并具有相同的转录方向。通过对这些基因组的比对发现，变异位点745个，占整个基因组的5.5%。其中，缺失/插入突变59个，碱基颠换105个，碱基转换581个。这些突变位点均匀地分布在整个线粒体基因组中。结论本研究提供了丰富的广州管圆线虫线粒体基因的突变位点，为构建种内鉴别诊断技术提供了基础。
목적：비교아국대륙지구엄주관원선충선립체기인조적다태성。방법재충군유전학연구기출상，선취7조자충진행선립체기인조적측정。근거엄주관원선충선립체기인조이지서렬（GQ398121）설계12대인물，진행PCR확증，병대목적편단진행측서화병접。이용다충생물학연건대측정적선립체기인조진행기인정위、결구도회제、핵감산급변이위점분석、진화관계분석。결과획득5개불동유전류형적선립체기인조。저사기인조적대소상사、결구상동，즉13491～13502대감기，함12개단백편마기인，2개핵당체기인，22개tRNA기인，2개교대적비편마구。상술기인긴밀지배렬재동일조DNA련상，병구유상동적전록방향。통과대저사기인조적비대발현，변이위점745개，점정개기인조적5.5%。기중，결실/삽입돌변59개，감기전환105개，감기전환581개。저사돌변위점균균지분포재정개선립체기인조중。결론본연구제공료봉부적엄주관원선충선립체기인적돌변위점，위구건충내감별진단기술제공료기출。
Objective To compare the diversity of mitochondrial genomes of Angiostrongylus cantonensis in the mainland of China. Methods According to the population genetic of A. cantonensis,seven female worms were selected to characterize the mi-tochondrial(MT)genomes. Twelve primer pairs based on known MT genome(GQ398121)were used for PCR. The target frag-ments were sequenced and aligned. The gene localization,genome structure,composition of nucleotide,distribution of variable sites,and phylogeny were analyzed by employing multiple softwares. Results Five distinct types were identified from seven com-plete MT genomes. They were similar in size and structure,i.e.,ranging 13 491-13 502 bp,including 12 protein-coding genes,2 ribosomal genes,22 tRNA genes,and 2 major non-coding regions. All the genes were localized at the same strand and had the same transcription direction. A total of 745 variable sites were identified,accounting for 5.5%. Among the variable sites,59 were deletion/insert mutations,105 transversions,and 581 transitions. The variable sites distributed evenly at the complete genome. Conclusion The study reveals the mutation profile in the whole MT genome of A. cantonensis and thus will facilitate the develop-ment of intraspecific differential diagnosis.