CAJ | 학술논문

目的：探究炎症调节基因miR-155和miR-146a及炎症相关基因在小鼠血吸虫病及吡喹酮治疗中的表达特征，为进一步阐明吡喹酮治疗血吸虫病的作用机制奠定基础。方法以BALB/c小鼠为研究对象，建立日本血吸虫感染的动物模型；小鼠随机分为4组：正常组、感染6周组、感染12周组和吡喹酮（PZQ，300 mg/kg 1次灌胃杀虫治疗）治疗组。以HE染色观察肝脏病理变化；以Real-time PCR检测肝脏miR-155和miR-146a及炎症相关基因TNF-α、IL-1β和IL-6的mRNA水平。结果感染6周组小鼠的肝脏miR-155、miR-146a及TNF-α、IL-1β和IL-6的mRNA水平均显著高于正常组和感染12周组（P＜0.05）；与感染12周组小鼠相比，PZQ治疗组小鼠肝脏虫卵肉芽肿反应减轻，且肝脏miR-155、miR-146a及TNF-α、IL-1β和IL-6的mRNA水平有所升高（P＜0.05）。结论本研究发现miR-155和miR-146a可能与血吸虫病肝脏炎症的发生发展有关，并且参与了吡喹酮对炎症的调节。
목적：탐구염증조절기인miR-155화miR-146a급염증상관기인재소서혈흡충병급필규동치료중적표체특정，위진일보천명필규동치료혈흡충병적작용궤제전정기출。방법이BALB/c소서위연구대상，건립일본혈흡충감염적동물모형；소서수궤분위4조：정상조、감염6주조、감염12주조화필규동（PZQ，300 mg/kg 1차관위살충치료）치료조。이HE염색관찰간장병리변화；이Real-time PCR검측간장miR-155화miR-146a급염증상관기인TNF-α、IL-1β화IL-6적mRNA수평。결과감염6주조소서적간장miR-155、miR-146a급TNF-α、IL-1β화IL-6적mRNA수평균현저고우정상조화감염12주조（P＜0.05）；여감염12주조소서상비，PZQ치료조소서간장충란육아종반응감경，차간장miR-155、miR-146a급TNF-α、IL-1β화IL-6적mRNA수평유소승고（P＜0.05）。결론본연구발현miR-155화miR-146a가능여혈흡충병간장염증적발생발전유관，병차삼여료필규동대염증적조절。
Objective To investigate the expression characteristics of miR-155 and miR-146a in mice with schistosomiasis and praziquantel(PZQ)treatment. Methods Totally 40 BABL/c mice were divided into 4 groups:a normal group,a 6W infect-ed group that were infected cutaneously with 10 Schistosoma japonicum cercariae for 6 weeks,a 12W infected group that were in-fected cutaneously with 10 Schistosoma japonicum cercariae for 12 weeks,and a praziquantel treated group that were infected cuta-neously with 10 Schistosoma japonicum cercariae and intragastrically administered with PZQ(300 mg/kg/day)for 1 day in 6 weeks post-infection and continuing surviving 6 weeks. The animals were sacrificed in 6 weeks and 12 weeks post-infection respectively. The left front lobe of each liver was stained with hematoxylin-eosin(HE)to detect pathological lesions. The levels of mRNA ex-pressions of miR-155,miR-146a and pro-inflammatory cytokines(TNF-α,IL-1βand IL-6)in the liver tissue were determined by using quantitative real-time PCR. Results The levels of mRNA expressions of miR-155,miR-146a and pro-inflammatory cyto-kines(TNF-α,IL-1βand IL-6)in the 6W infected mice were significantly higher than those of the normal mice and of the 12W in-fected mice. Compared with the 12W infected mice,the inflammation response of liver egg granuloma in the PZQ-treated mice was ameliorated. Furthermore,there was a marked increase in the levels of mRNA expressions of miR-155,miR-146a and three pro-in-flammatory cytokines in the PZQ-treated mice compared to the 12W infected mice. Conclusion miR-155 and miR-146a may play a role in schistosomiasis liver inflammation response and the inflammation regulation of praziquantel treatment.