中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2014年
5期
398-401
,共4页
林桂平%郑巧%林创杰%邓若梅子%冯蔚姣%胡航通%刘伟%向秋玲
林桂平%鄭巧%林創傑%鄧若梅子%馮蔚姣%鬍航通%劉偉%嚮鞦玲
림계평%정교%림창걸%산약매자%풍위교%호항통%류위%향추령
二十二碳六烯酸%抑郁症%海马神经元%凋亡%caspase-3
二十二碳六烯痠%抑鬱癥%海馬神經元%凋亡%caspase-3
이십이탄륙희산%억욱증%해마신경원%조망%caspase-3
Docosahexaenoic acid%Depression%Hippocampal neuron%Apoptosis%Caspase-3
目的 观察二十二碳六烯酸(docosahexaenoic acid,DHA)对抑郁症的治疗作用及其对海马神经元caspase-3表达的影响.方法 50只SD大鼠随机分为5组(空白对照组,抑郁模型组,氟西汀组,DHA组,氟西汀与DHA联合用药组),每组10只,连续21 d每天给予不同刺激,建立慢性应激不可预见抑郁大鼠模型.21 d后强迫游泳实验进行行为学检测,HE染色检测海马CA1、CA2、CA3区锥体细胞的形态学变化.荧光实时定量PCR和Western Blot检测海马神经元caspase-3的变化.结果 行为学检测显示,抑郁模型组、DHA组、DHA联合氟西汀组大鼠在水中的游泳不动时间分别为(113.33±9.50)s、(81.67±7.68)s和(73.00±8.54)s.与抑郁模型组相比,DHA组和DHA联合氟西汀组大鼠在水中游泳不动时间减少(t=8.164,9.855,P＜0.01).HE染色切片可见DHA使大鼠海马CA1、CA2、CA3区神经元数量部分恢复,排列致密,可减少海马神经元凋亡.qPCR和WB结果显示DHA单独或联合氟西汀可抑制海马神经元caspase-3 mRNA和蛋白的表达.结论 DHA对慢性应激不可预见抑郁模型大鼠有治疗及辅助治疗作用,其机制与DHA减少海马神经元凋亡、下调caspase-3有关.
目的 觀察二十二碳六烯痠(docosahexaenoic acid,DHA)對抑鬱癥的治療作用及其對海馬神經元caspase-3錶達的影響.方法 50隻SD大鼠隨機分為5組(空白對照組,抑鬱模型組,氟西汀組,DHA組,氟西汀與DHA聯閤用藥組),每組10隻,連續21 d每天給予不同刺激,建立慢性應激不可預見抑鬱大鼠模型.21 d後彊迫遊泳實驗進行行為學檢測,HE染色檢測海馬CA1、CA2、CA3區錐體細胞的形態學變化.熒光實時定量PCR和Western Blot檢測海馬神經元caspase-3的變化.結果 行為學檢測顯示,抑鬱模型組、DHA組、DHA聯閤氟西汀組大鼠在水中的遊泳不動時間分彆為(113.33±9.50)s、(81.67±7.68)s和(73.00±8.54)s.與抑鬱模型組相比,DHA組和DHA聯閤氟西汀組大鼠在水中遊泳不動時間減少(t=8.164,9.855,P＜0.01).HE染色切片可見DHA使大鼠海馬CA1、CA2、CA3區神經元數量部分恢複,排列緻密,可減少海馬神經元凋亡.qPCR和WB結果顯示DHA單獨或聯閤氟西汀可抑製海馬神經元caspase-3 mRNA和蛋白的錶達.結論 DHA對慢性應激不可預見抑鬱模型大鼠有治療及輔助治療作用,其機製與DHA減少海馬神經元凋亡、下調caspase-3有關.
목적 관찰이십이탄륙희산(docosahexaenoic acid,DHA)대억욱증적치료작용급기대해마신경원caspase-3표체적영향.방법 50지SD대서수궤분위5조(공백대조조,억욱모형조,불서정조,DHA조,불서정여DHA연합용약조),매조10지,련속21 d매천급여불동자격,건립만성응격불가예견억욱대서모형.21 d후강박유영실험진행행위학검측,HE염색검측해마CA1、CA2、CA3구추체세포적형태학변화.형광실시정량PCR화Western Blot검측해마신경원caspase-3적변화.결과 행위학검측현시,억욱모형조、DHA조、DHA연합불서정조대서재수중적유영불동시간분별위(113.33±9.50)s、(81.67±7.68)s화(73.00±8.54)s.여억욱모형조상비,DHA조화DHA연합불서정조대서재수중유영불동시간감소(t=8.164,9.855,P＜0.01).HE염색절편가견DHA사대서해마CA1、CA2、CA3구신경원수량부분회복,배렬치밀,가감소해마신경원조망.qPCR화WB결과현시DHA단독혹연합불서정가억제해마신경원caspase-3 mRNA화단백적표체.결론 DHA대만성응격불가예견억욱모형대서유치료급보조치료작용,기궤제여DHA감소해마신경원조망、하조caspase-3유관.
Objective To explore the therapeutic effects of docosahexaenoic acid (DHA) on depression,and its influence on the expression of caspase-3 in hippocampal neuron.Methods A total of 50 SD rats were randomly divided into 5 groups (control,model,fluoxetine,DHA,combination group),10 rats in each group.SD rats were given different stimuli for 21 days to set up unforeseeable chronic stress depression model.After 21 days,the changes of behaviors in forced swimming test and morphology change in CA1,CA2 and CA3 in hippocampal neurons were observed.The change of caspase-3 in hippocampal neuron were detected by real-time PCR and western blot.Results Behavioral results showed that the swimming immobility time in depression model group,DHA group and combination group were respectively(113.33±9.50) s,(81.67±7.68) s and(73.00±8.54) s.Compared with depression model group,the DHA group and DHA combined with fluoxetine group obviously reduced the swimming immobility time of rats(t=8.164,9.855,P＜0.01).HE stain result showed DHA could partly recovered the number of hippocampal neurons in CA1,CA2 and CA3 and reduced apoptosis.qPCR and WB results showed DHA alone or DHA combined with fluoxetine could inhibit mRNA and protein level of caspase-3 in hippocampal neurons.Conclusion DHA can improve depression to a certain extent in a rat model of unforeseeable chronic stress depression,which might work through inhibition of neuron apoptosis and caspase-3 expression in hippocampal neurons.
