CAJ | 학술논문

目的：确定赤桉叶中主要GC/MS峰所代表的化学成分以及其对杀螺作用的贡献，探索一条从天然产物中寻找有效成分的快捷途径。方法在同一地点连续采集12个月的赤桉树叶，并采用二氯甲烷提取；利用GC/MS技术分析其化学成分；采用室内浸杀法进行杀螺药效实验并计算LC50；利用灰色系统理论建模软件对12个月赤桉叶提取物的主要成分及其相应提取物的杀螺活性作灰色关联分析。结果赤桉树叶的二氯甲烷提取物均表现出较好的杀螺活性，尤以6月和7月采集的样品杀螺作用最强，其LC50分别为0.257 mg/L和0.242 mg/L，而1月和12月的样品杀螺作用最弱，LC50分别为6.802 mg/L和5.406 mg/L。GC/MS分析鉴定了16个主要化学成分，多为单萜及倍半萜类化合物；与杀螺活性的灰色相对关联度均>0.5，其中5个成分的灰色关联度>0.9，分别为4，4，8-三甲基[6.3.1.0（1，5）]三环十二烷-2，9-二醇、（-）-桉油烯醇、（-）-桉油烯醇的同分异构体或类似物、1，8-桉叶油素、喇叭茶醇。结论赤桉叶二氯甲烷提取物中多个成分与杀螺活性均表现出了较好的关联度，提示其杀灭钉螺作用可能是多种化学成分共同作用的结果。
목적：학정적안협중주요GC/MS봉소대표적화학성분이급기대살라작용적공헌，탐색일조종천연산물중심조유효성분적쾌첩도경。방법재동일지점련속채집12개월적적안수협，병채용이록갑완제취；이용GC/MS기술분석기화학성분；채용실내침살법진행살라약효실험병계산LC50；이용회색계통이론건모연건대12개월적안협제취물적주요성분급기상응제취물적살라활성작회색관련분석。결과적안수협적이록갑완제취물균표현출교호적살라활성，우이6월화7월채집적양품살라작용최강，기LC50분별위0.257 mg/L화0.242 mg/L，이1월화12월적양품살라작용최약，LC50분별위6.802 mg/L화5.406 mg/L。GC/MS분석감정료16개주요화학성분，다위단첩급배반첩류화합물；여살라활성적회색상대관련도균>0.5，기중5개성분적회색관련도>0.9，분별위4，4，8-삼갑기[6.3.1.0（1，5）]삼배십이완-2，9-이순、（-）-안유희순、（-）-안유희순적동분이구체혹유사물、1，8-안협유소、나팔다순。결론적안협이록갑완제취물중다개성분여살라활성균표현출료교호적관련도，제시기살멸정라작용가능시다충화학성분공동작용적결과。
Objective To determine the contributions of main chemical compositions of extracts of Eucalyptus camaldulensis represented by GC/MS elute peaks to the molluscicidal activities,and explore a shortcut of looking for the effective components from natural products. Methods E. camaldulensis leaves were collected consecutively in 12 months at the same place,extracted with dichloromethane,analyzed by GC/MS,and their LC50(s)of molluscicidal activities were tested according to the method rec-ommended by WHO. The correlation of the main components in 12 extracts and their molluscicidal activities were analyzed by the grey relative correlation analysis model with software GTMS 3.0. Result All the dichloromethane extracts of eucalyptus leaves showed excellent molluscicidal activities with the highest LC50 of 0.257mg/L and 0.242mg/L for the samples in June and July and the lowest LC50 of 6.802 mg/L and 5.406 mg/L in December and January respectively. The structures of 16 main chemical compo-nents were elucidated by GC/MS and NIST Mass Spectral Library,most of which were monoterpenes and sesquiterpenoids. The gray correlation coefficients with activity were all over 0.5,the first five over 0.9 were 4,4,8-Trimethyltricyclo[6.3.1.0(1,5)]do-decane-2,9-diol,(-)-Spathulenol,a structural isomer of(-)-Spathulenol,Eucalyptol and Ledol. Conclusion The most main in-gredients in the dichloromethane extracts of E. camaldulensis leaves show good correlations with the molluscicidal activity,which suggests that the molluscicidal role is synergistically played by the multiple components together.