CAJ | 학술논문

目的:观察血红素氧合酶-1 (heme oxygenase-1,HO-1)抑制剂对高氧暴露早产鼠视网膜血管内皮生长因子(vascular endothelial growth factor,VEGF)和色素上皮衍生因子(pigment epithelium-derived factor,PEDF)表达的影响.方法:将144只3日龄早产大鼠随机分为A高氧组、B干预组、C空气组、0空气+舒血宁组,以免疫组织化学法检测各组早产鼠的视网膜HO-1、PEDF和VEGF表达水平.结果:C、D组HO-1、PEDF、VEGF均呈低表达,未见或罕见突破内界膜的内皮细胞核；与C、D组相比,A组突破内界膜的内皮细胞核明显增多,HO-1 、PEDF及VEGF表达的表达均显著增加,(P分别＜0.001,0.001,0.001),与A组相比,B组突破内界膜的内皮细胞核明显减少,HO-1、PEDF及VEGF的表达均显著下降(P分别＜0.05,0.001,0.05)；视网膜PEDF的表达与VEGF的表达、视网膜PEDF的表达与突破视网膜内界膜内皮细胞核数呈负相关、VEGF、HO-1的表达与突破视网膜内界膜内皮细胞核数呈正相关(P分别＜0.01,0.01,0.05).结论:高氧暴露早产鼠视网膜内可观察到HO-1 、VEGF和PEDF的高表达,舒血宁通过下调高氧暴露早产鼠视网膜HO-1、VEGF和PEDF的表达,对高氧性视网膜病有一定的保护作用.
목적:관찰혈홍소양합매-1 (heme oxygenase-1,HO-1)억제제대고양폭로조산서시망막혈관내피생장인자(vascular endothelial growth factor,VEGF)화색소상피연생인자(pigment epithelium-derived factor,PEDF)표체적영향.방법:장144지3일령조산대서수궤분위A고양조、B간예 조、C공기조、0공기+서혈저조,이면역조직화학법검측각조조산서적시망막HO-1、PEDF화VEGF표체수평.결과:C、D조HO-1、PEDF、VEGF균정저표체,미견혹한견돌파내계막적내피세포핵；여C、D조상비,A조돌파내계막적내피세포핵명현증다,HO-1 、PEDF급VEGF표체적표체균현저증가,(P분별＜0.001,0.001,0.001),여A조상비,B조돌파내계막적내피세포핵명현감소,HO-1、PEDF급VEGF적표체균현저하강(P분별＜0.05,0.001,0.05)；시망막PEDF적표체여VEGF적표체、시망막PEDF적표체여돌파시망막내계막내피세포핵수정부상관、VEGF、HO-1적표체여돌파시망막내계막내피세포핵수정정상관(P분별＜0.01,0.01,0.05).결론:고양폭로조산서시망막내가관찰도HO-1 、VEGF화PEDF적고표체,서혈저통과하조고양폭로조산서시망막HO-1、VEGF화PEDF적표체,대고양성시망막병유일정적보호작용.