山西中医学院学报
山西中醫學院學報
산서중의학원학보
JOURNAL OF SHANXI COLLEGE OF TRADITIONAL CHINESE MEDICINE
2013年
5期
25-28,30
,共5页
涂颖秋%蒋杉杉%李玉清%黄绳武
塗穎鞦%蔣杉杉%李玉清%黃繩武
도영추%장삼삼%리옥청%황승무
鸦胆子油%蟾酥%复方脂质体%制备工艺
鴉膽子油%蟾酥%複方脂質體%製備工藝
아담자유%섬소%복방지질체%제비공예
oleum fructus bruceae%Veneum Bufonis%compound liposomes%preparation process
目的:研究复方鸦胆子油脂质体的处方筛选及制备工艺。方法:采用气相色谱法测定脂质体包封率。以脂质体包封率为主要指标,对制备工艺中可能影响脂质体性质的工艺参数和处方因素进行单因素考察和正交设计,优化制备工艺。用不同冻干保护剂进行冷冻干燥,以筛选合适的保护剂。测定Zeta电位、粒径大小及分布。结果:采用乙醇注入法制备复方鸦胆子油脂质体;最佳制备工艺为磷脂浓度为1.5%,磷脂胆固醇质量比为5∶1,油水两相体积比为1∶10,甘露醇∶蔗糖∶磷脂质量比为1∶1∶1,水相介质为蒸馏水,温度为50℃,搅拌速度为30 rpm。最佳处方制备的平均包封率为91.9%, Zeta电位为-32.1 mV,平均粒径为170.3 nm。结论:该实验制备的复方鸦胆子油脂质体具有包分率高、稳定性好、表面带负电等特点,为进一步研究复方鸦胆子油脂质体奠定了基础。
目的:研究複方鴉膽子油脂質體的處方篩選及製備工藝。方法:採用氣相色譜法測定脂質體包封率。以脂質體包封率為主要指標,對製備工藝中可能影響脂質體性質的工藝參數和處方因素進行單因素攷察和正交設計,優化製備工藝。用不同凍榦保護劑進行冷凍榦燥,以篩選閤適的保護劑。測定Zeta電位、粒徑大小及分佈。結果:採用乙醇註入法製備複方鴉膽子油脂質體;最佳製備工藝為燐脂濃度為1.5%,燐脂膽固醇質量比為5∶1,油水兩相體積比為1∶10,甘露醇∶蔗糖∶燐脂質量比為1∶1∶1,水相介質為蒸餾水,溫度為50℃,攪拌速度為30 rpm。最佳處方製備的平均包封率為91.9%, Zeta電位為-32.1 mV,平均粒徑為170.3 nm。結論:該實驗製備的複方鴉膽子油脂質體具有包分率高、穩定性好、錶麵帶負電等特點,為進一步研究複方鴉膽子油脂質體奠定瞭基礎。
목적:연구복방아담자유지질체적처방사선급제비공예。방법:채용기상색보법측정지질체포봉솔。이지질체포봉솔위주요지표,대제비공예중가능영향지질체성질적공예삼수화처방인소진행단인소고찰화정교설계,우화제비공예。용불동동간보호제진행냉동간조,이사선합괄적보호제。측정Zeta전위、립경대소급분포。결과:채용을순주입법제비복방아담자유지질체;최가제비공예위린지농도위1.5%,린지담고순질량비위5∶1,유수량상체적비위1∶10,감로순∶자당∶린지질량비위1∶1∶1,수상개질위증류수,온도위50℃,교반속도위30 rpm。최가처방제비적평균포봉솔위91.9%, Zeta전위위-32.1 mV,평균립경위170.3 nm。결론:해실험제비적복방아담자유지질체구유포분솔고、은정성호、표면대부전등특점,위진일보연구복방아담자유지질체전정료기출。
Objective:To optimize the prescription and preparation of compound oleum fructus bruceae liposomes. Methods:The encapsulation efficiency of liposomes was examined by Gas Chromatography. Orthogonal design was adopted and encap-sulation efficiency of liposomes was taken as index in observation. Formulation and preparation factors which may influence properties of liposomes were studied. Result:Compound oleum fructus bruceae liposomes was prepared by ethanol injection method. The optimum preparation conditions were as follows:the concentration of the soybean lecithin was 1.5%,ratio of lecithin to cholesterol was 5∶1,volume ratio of water-oil was 10∶1,the best proportion for mannitol,sucrose,lecithin was 1∶1∶1, the aqueous phase was distilled water,the temperature was 50℃,and the stirring speed was 30 rpm. The average encapsula-tion efficiency was 91.9%. The Zeta potential of the liposomes was -32.1 mV and the average size was 170.3 nm. Conclu-sion:The formulation and preparation process were practical to prepare the compound liposomes with good encapsulation efficiency and stability,negative charged surface. It was valuable to be further study for oleum fructus bruceae liposomes.