CAJ | 학술논문

利用MS培养液研究了不同浓度氯化钙对马铃薯试管薯的产量、质量、薯内钙含量及生理病害的影响。结果表明，MS培养液中不添加钙时，试管薯的结薯数量、薯重及大薯率大幅度降低，没有＞100 mg的试管薯，薯表面出现明显的黄褐色粗糙斑块，个别薯块现水浸状腐烂，腐烂率2.21％。薯内干物质、淀粉、可溶性糖和蛋白质含量也较正常钙用量下分别降低了36.8％、41.39％、10.7％和39.43％，丙二醛含量上升。4℃下贮藏6个月，薯皮失水皱缩严重，平均病害率达53.77％；室温下放置3周，发芽率25.53％，芽生长缓慢；MS培养液中添加不同浓度的氯化钙，茎段结薯数和薯重随钙浓度的增加呈先增加后降低的变化趋势，大薯率和薯皮表面光泽度呈上升趋势，与正常钙水平相比，薯重增加了2.7％47.86％、结薯数增加了-11.49％22.64％、大薯率14.43％26.08％。薯内干物质、淀粉、可溶性糖和蛋白质含量的变化均随钙浓度的增加呈先上升后下降的趋势，并较正常钙用量下增加了4.43％8.62％（干物质）、6.79％16.49％（淀粉）、3.89％6.31％（可溶性糖）和4.63％8.29％（蛋白质）。薯内钙含量大幅度上升，平均值为177267μg/g （最高值为9 mmol/L处理），薯皮光滑无水浸状腐烂。4℃下贮藏6个月，薯皮正常，生理病害率随薯内钙含量升高呈下降趋势，病害率为1％8.9％，决定系数R2＝0.3812，且试管薯芽的萌发和生长能力正常。确定MS培养液中添加9 mmol/L CaCl2是“新大坪”品种试管薯数量和质量的最优处理。
이용MS배양액연구료불동농도록화개대마령서시관서적산량、질량、서내개함량급생리병해적영향。결과표명，MS배양액중불첨가개시，시관서적결서수량、서중급대서솔대폭도강저，몰유＞100 mg적시관서，서표면출현명현적황갈색조조반괴，개별서괴현수침상부란，부란솔2.21％。서내간물질、정분、가용성당화단백질함량야교정상개용량하분별강저료36.8％、41.39％、10.7％화39.43％，병이철함량상승。4℃하저장6개월，서피실수추축엄중，평균병해솔체53.77％；실온하방치3주，발아솔25.53％，아생장완만；MS배양액중첨가불동농도적록화개，경단결서수화서중수개농도적증가정선증가후강저적변화추세，대서솔화서피표면광택도정상승추세，여정상개수평상비，서중증가료2.7％47.86％、결서수증가료-11.49％22.64％、대서솔14.43％26.08％。서내간물질、정분、가용성당화단백질함량적변화균수개농도적증가정선상승후하강적추세，병교정상개용량하증가료4.43％8.62％（간물질）、6.79％16.49％（정분）、3.89％6.31％（가용성당）화4.63％8.29％（단백질）。서내개함량대폭도상승，평균치위177267μg/g （최고치위9 mmol/L처리），서피광활무수침상부란。4℃하저장6개월，서피정상，생리병해솔수서내개함량승고정하강추세，병해솔위1％8.9％，결정계수R2＝0.3812，차시관서아적맹발화생장능력정상。학정MS배양액중첨가9 mmol/L CaCl2시“신대평”품충시관서수량화질량적최우처리。
This study determined the microtuber yield , quality, calcium content and physiological disorder of potato in response to the various concentrations of CaCl 2.Potato was cultivated in the liquid MS medium with various concentrations of CaCl 2.The MS medium with zero calcium reduces the number of microtubers , tuber weight and the percent of large tubers .With the calcium-free medium , no microtubers larger than 100 mg are found , their tuber skin is yellowish brown plaques , and some tubers exhibits water-soaked decay with decay rate being 2.21%. The dry weight, starch, soluble sugar and protein contents in the microtubers cultured in the calcium -free medium are decreased by 36.8%, 41.39%, 10.7% and 39.43%, respectively , while the malonaldehyde content is increased.After being stored for six months under 4oC, the tuber skin shows the sign of dehydration , with physiological disorder averaging 53.77%.When stored at room temperature for three weeks , the germination rate is only 25.53%with a slow growth .With the addition of calcium to the MS medium , the number of microtubers per stem and tuber weight are increased with the increase of calcium concentrations and then decreased at higher concentrations .The percent of large tubers and the tuber skin glossiness are increased with the increase of calcium concentrations .Compared to the microtubers grown in the standard MS medium , the dry matter of microtubers in the higher calcium medium is increased by 4.43%-8.62%, the starch content is increased by 6.79%-16.49%, the soluble sugar content is increased by 2.7%-47.9%, and protein concentration is increased by 4.63%-8.29%.Average tuber calcium content is increased significantly , reaching the range from 177μg/g to 267 μg/g, and the tuber skin does not show the sign of decay .After being stored for six months under 4℃, the tuber skin is normal, and the physiological disorder rate is reduced to 1%-8.9% with the increase of calcium concentration , and the coefficient of determination is 0.3812.Calcium concentrations do not affect the germination ability and bud growth .We conclude that the addition of 9 mmol/L of CaCl 2 in the MS medium is the best practice for the potato cultivar ‘Xindaping ’ to improve its yield and quality .