CAJ | 학술논문

为探索钙对化学诱抗剂水杨酸（SA）、茉莉酸甲酯（MeJA）、龙胆酸（GA）和β-氨基丁酸（BABA）诱导番茄木质素合成的影响，在番茄六叶期用上述诱抗剂分别处理第3叶片，然后喷施CaCl 2、蒸馏水、乙二醇-双-（2-氨基乙醚）四乙酸（ EGTA）或LaCl3，处理后0、1、2、3、5 d测定各处理番茄第3叶片（诱导叶）和第5叶片（非诱导叶）中木质素含量的变化。结果表明：（1）用上述化学诱抗剂处理番茄第3叶片，诱导叶及其上位非诱导叶中木质素含量均升高；（2）外源Ca2＋进一步促进上述诱抗剂诱导番茄叶片中木质素含量的增加；而Ca2＋螯合剂EGTA和质膜钙通道抑制剂LaCl3，则不同程度地抑制这些诱抗剂对木质素含量的诱导作用。这些结果表明，钙对上述4种诱抗剂诱导番茄叶片中木质素的合成具有正调控作用。
위탐색개대화학유항제수양산（SA）、말리산갑지（MeJA）、룡담산（GA）화β-안기정산（BABA）유도번가목질소합성적영향，재번가륙협기용상술유항제분별처리제3협편，연후분시CaCl 2、증류수、을이순-쌍-（2-안기을미）사을산（ EGTA）혹LaCl3，처리후0、1、2、3、5 d측정각처리번가제3협편（유도협）화제5협편（비유도협）중목질소함량적변화。결과표명：（1）용상술화학유항제처리번가제3협편，유도협급기상위비유도협중목질소함량균승고；（2）외원Ca2＋진일보촉진상술유항제유도번가협편중목질소함량적증가；이Ca2＋오합제EGTA화질막개통도억제제LaCl3，칙불동정도지억제저사유항제대목질소함량적유도작용。저사결과표명，개대상술4충유항제유도번가협편중목질소적합성구유정조공작용。
The effects of calcium on synthesis of lignin induced by chemical elicitors in tomato were investigated in this study.The 3rd leaves were smeared with chemical elicitors:salicylic acid(SA), Methyl jasmonate(MeJA),β-aminobutyric acid ( BABA ) and gentisic acid ( GA ) separately at the six-leaf stage of tomato .After the third leaves dried under room temperature , all the tomato plants were sprayed with CaCl 2 , distilled water , EGTA and LaCl3 in turn.The contents of lignin in the third leaves ( induced ) and the fifth leaves ( un-induced ) were determined in 0, 1, 2, 3 and 5 days after treatments.The results show that:(1) The lignin content in both the induced leaves and un-induced leaves increased after treated with chemical elicitors .( 2 ) The lignin synthesis in treated tomato leaves were further promoted by exterior Ca 2+, but inhibited by Ca 2+ chelator EGTA and the plasmalemma Ca 2+ channel blocker La 3 + in different levels .These results indicated that Ca 2+play a positive regulation role in synthesis of lignin in tomato leaves induced by the above four chemical elicitors .