CAJ | 학술논문

脲酶催化尿素的水解生成形成铵根离子和碳酸根离子，具有广泛的应用价值。以巴氏芽孢八叠球菌ATCC11859为出发菌株，通过亚硝基胍诱变，得到高产脲酶突变株。其中菌株B-20的脲酶活性最高，为28．4 mmol/min，较出发菌株提高了207％。对该菌株接种量、氮源种类培养条件进行优化，结果表明接种量为10％，且采用大豆粉为氮源时，脲酶活性最高为32．9 mmol/min。对脲酶高产菌株的选育表明该方法是一种行之有效的方法。
뇨매최화뇨소적수해생성형성안근리자화탄산근리자，구유엄범적응용개치。이파씨아포팔첩구균ATCC11859위출발균주，통과아초기고유변，득도고산뇨매돌변주。기중균주B-20적뇨매활성최고，위28．4 mmol/min，교출발균주제고료207％。대해균주접충량、담원충류배양조건진행우화，결과표명접충량위10％，차채용대두분위담원시，뇨매활성최고위32．9 mmol/min。대뇨매고산균주적선육표명해방법시일충행지유효적방법。
Urease catalyzed hydrolysis of urea formed ammonium ions and carbonate ions. It has extensive application value. The strain of S. pasteurii ATCC11859 was screened through NTG ( N-methyl-N″-nitroso-N-nitrosoguanidine) mutation and a high yielding urease mutant was obtained. Among which strain B-20 gave the highest urease activity of 28.37 mmol/min. This was increased 207% of the original strain. The strain inoculation amount and culture conditions of nitrogen source type were optimized. The results showed that the inoculation quantity was 10%, and using soybean meal as nitrogen source could achieve maximum urease activity as high as 32. 9 mmol/min. This indicated that the technology is effective method for breeding high-yield urease strain.