CAJ | 학술논문

目的检测乳腺癌新辅助化疗前后凝溶胶蛋白(GSN)前体的变化,以期发现预测乳腺癌化疗耐药的血清蛋白标志物.方法以Ⅲ期乳腺癌新辅助化疗患者为研究对象,分别留取患者新辅助化疗前后的血清标本,采用双向凝胶电泳得到化疗前后的凝胶图谱,应用凝胶图像分析软件进行分析,筛选化疗前后差异表达蛋白质点,应用基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)技术对差异蛋白质胶点进行质谱鉴定,筛选出血清差异蛋白质,其中包括GSN前体.应用Western印迹法验证其在化疗前后的变化趋势.结果新辅助化疗后耐药组的蛋白表达集中于相对低丰度区域,共筛选和鉴定出13个差异位点,其中上调蛋白7个,下调蛋白6个.其中GSN前体水平下调,Western印迹法验证结果与蛋白质组学结果变化趋势一致.结论 GSN前体有可能作为预测乳腺癌化疗耐药性的候选血清标志物.
목적 검측유선암신보조화료전후응용효단백(GSN)전체적변화,이기발현예측유선암화료내약적혈청단백표지물.방법 이Ⅲ기유선암신보조화료환자위연구대상,분별류취환자신보조화료전후적혈청표본,채용쌍향응효전영득도화료전후적응효도보,응용응효도상분석연건진행분석,사선화료전후차이표체단백질점,응용기질보조격광해석전리비행시간질보(MALDI-TOF-MS)기술대차이단백질효점진행질보감정,사선출혈청차이단백질,기중포괄GSN전체.응용Western인적법험증기재화료전후적변화추세.결과 신보조화료후내약조적단백표체집중우상대저봉도구역,공사선화감정출13개차이위점,기중상조단백7개,하조단백6개.기중GSN전체수평하조,Western인적법험증결과여단백질조학결과변화추세일치.결론 GSN전체유가능작위예측유선암화료내약성적후선혈청표지물.
Objective To detect the change of precursor protein of sol in breast cancer before and after the neoadjuvant chemotherapy,so as to find the serum protein marker of chemotherapy drug resistance in breast cancer.Methods Breast cancer patients with Ⅲ period neoadjuvant chemotherapy were enrolled in the study.The serum specimens of the patients were obtained.Two-way gel electrophoresis was used to get the gel map before and after chemotherapy,then the image map was analyzed by gel analysis software.The differentially expressed proteins point was screened before and after chemotherapy.Serum protein differences were identified with matrix auxiliary laser disi ntegrate series time of flight mass spectrometer (MALDI-TOF-MS),including coagulation sol precursor protein.Western blot was used to test the variation trend of them before and after chemotherapy.Results Coagulation sol precursor protein levels fell after neoadjuvant chemotherapy in resistance group.The variation trend of the results of western blot and that of the proteomics were consistent.Conclusion Coagulation sol precursor protein can be as the candidate serum marker of chemotherapy drug resistance in breast cancer.