CAJ | 학술논문

目的：探讨小睑裂综合征（BPES）患者血液中FOXL2，SOX14及BPESC13个基因mRNA的相对表达水平与BPES的相关性，以进一步研究可能存在的发病机制。方法收集小睑裂综合征患者（ A组30例）和正常人（ B组30例）的外周静脉血液，TRIzol提取总RNA，反转录为cDNA。应用荧光定量PCR技术检测FOXL2， BPESC1，SOX14的相对表达量，采用配对比较 t 检验法和 Livak（2-ΔΔCt）法分析两组数据及其差异。结果FOXL2基因mRNA的在A，B两组间ΔCt差异具有统计学意义（P＝0．023＜0．05），且A组大于B组；BPESC1差异亦有统计学意义（P＝0．008＜0．05），且A组小于B组；SOX14差异无统计学意义（P＝0．157＞0．05）。可以认为A组FOXL2基因的表达水平低于B组，BPESC1基因的表达水平高于B组，SOX14基因两组表达无差别。结论 FOXL2，BPESC1与BPES具有一定相关性，FOXL2低表达以及BPESC1的高表达可能与BPES的发病相关。
목적：탐토소검렬종합정（BPES）환자혈액중FOXL2，SOX14급BPESC13개기인mRNA적상대표체수평여BPES적상관성，이진일보연구가능존재적발병궤제。방법수집소검렬종합정환자（ A조30례）화정상인（ B조30례）적외주정맥혈액，TRIzol제취총RNA，반전록위cDNA。응용형광정량PCR기술검측FOXL2， BPESC1，SOX14적상대표체량，채용배대비교 t 검험법화 Livak（2-ΔΔCt）법분석량조수거급기차이。결과FOXL2기인mRNA적재A，B량조간ΔCt차이구유통계학의의（P＝0．023＜0．05），차A조대우B조；BPESC1차이역유통계학의의（P＝0．008＜0．05），차A조소우B조；SOX14차이무통계학의의（P＝0．157＞0．05）。가이인위A조FOXL2기인적표체수평저우B조，BPESC1기인적표체수평고우B조，SOX14기인량조표체무차별。결론 FOXL2，BPESC1여BPES구유일정상관성，FOXL2저표체이급BPESC1적고표체가능여BPES적발병상관。
Objective To detect mRNA expression of FOXL2,SOX14 and BPESC1 from BPES patients and define the correla-tion between the exoression and BPES ,in order to expore the possible pathogenesis of BPES .Methods The peripheral venous blood was col-lected from 30 cases of BFPS patients(Group A)and 30 cases of normal individuals(Group B).RNA was extracted by TRIzol and then was transcripted to cDNA reversely .Real -time fluorescence quantitative PCR was applied to detect the relative mRNA expression levels of FOXL2,SOX14 and BPESC1 in blood from Group A and Group B .Two paired sample test and Livak (2 -ΔΔCt ) method were used to analyze the differences between two data sets .Results Between groups A and B,PFOXL2 =0.023 <0.05 and Group A was higher than Group B;PBPESC1 =0.008<0.05 and Group A was lower than Group B;PSOX14 =0.157>0.05,there was no statistical differences .It indicated that FOXL2 expression in Group A was lower than that in Group B ,while BPESC1 expression in Group A was higher than that in Group B .Con-clusion FOXL2 and BPESC1 are associated with BPES ,and low expression of FOXL2 and high expression of BPESC 1 might be related to BPES pathogenesis .