CAJ | 학술논문

目的：探讨白芍总苷(TGP)对人永生化角质形成细胞（HaCaT细胞）增生及分泌细胞间黏附分子(ICAM)-1的影响，探讨可能涉及的信号传导通路。方法用500 U/ml干扰素(IFN)-γ诱导HaCaT细胞，不同浓度TGP（0.5～312.5μg/ml）作用于HaCaT细胞。四甲基偶氮唑盐(MTT)法观察TGP对HaCaT细胞增生活性的影响。酶联免疫吸附试验（ELISA）及荧光免疫组化法检测HaCaT细胞表达ICAM-1的水平。结果 TGP在低浓度（0.5～25.0μg/ml）时对HaCaT细胞增生活性有促进作用，在高浓度（62.5～125.0μg/ml）时对HaCaT细胞增生活性呈抑制作用。0.5～25.0μg/ml TGP可显著降低HaCaT细胞表达ICAM-1的水平（P＜0.05，P＜0.01）。结论 TGP对IFN-γ上调HaCaT细胞分泌ICAM-1有显著的抑制作用，可能是TGP抗炎作用机制之一。
목적：탐토백작총감(TGP)대인영생화각질형성세포（HaCaT세포）증생급분비세포간점부분자(ICAM)-1적영향，탐토가능섭급적신호전도통로。방법용500 U/ml간우소(IFN)-γ유도HaCaT세포，불동농도TGP（0.5～312.5μg/ml）작용우HaCaT세포。사갑기우담서염(MTT)법관찰TGP대HaCaT세포증생활성적영향。매련면역흡부시험（ELISA）급형광면역조화법검측HaCaT세포표체ICAM-1적수평。결과 TGP재저농도（0.5～25.0μg/ml）시대HaCaT세포증생활성유촉진작용，재고농도（62.5～125.0μg/ml）시대HaCaT세포증생활성정억제작용。0.5～25.0μg/ml TGP가현저강저HaCaT세포표체ICAM-1적수평（P＜0.05，P＜0.01）。결론 TGP대IFN-γ상조HaCaT세포분비ICAM-1유현저적억제작용，가능시TGP항염작용궤제지일。
Objective To evaluate the effects of total glucosides of paeony (TGP) on cell proliferation of human HaCaT keratinocytes and the expression of ICAM-1, and explore the signaling pathways which may be involved in the process. Methods HaCaT cells were stimulated by 500 U/mL IFN-γ, then incubated with TGP of various concentrations (0.5 to 312.5 μg/ml). MTT assay was performed to detect the cell proliferation of HaCaT cells. The expression of ICAM-1 in HaCaT cells was evaluated by enzyme linked immunosorbent assay (ELISA) and lfuorescent immunohistochemical technology. Results The proliferation of HaCaT cells was promoted by TGP of low concentrations (0.5 to 25.0μg/ml), but inhibited by TGP of high concentration (62.5 to 125.0μg/ml). TGP of low concentrations (0.5 to 25.0μg/mL) signiifcantly reduced the expression of ICAM-1. Conclusion TGP signiifcantly inhibited the upregulated ICAM-1 secretion of HaCaT cells which induced by IFN-γ, that may be one of the anti-inlfammatory mechanism of TGP.