实验与检验医学
實驗與檢驗醫學
실험여검험의학
EXPERIMENTAL AND LABORATORY MEDICINE
2013年
6期
549-551
,共3页
分析前%血管紧张素Ⅱ%肾素活性
分析前%血管緊張素Ⅱ%腎素活性
분석전%혈관긴장소Ⅱ%신소활성
Preanalytical%Plasma angiotensinⅡ%Plasma renin activity
目的：研究血管紧张素Ⅱ(AⅡ)和肾素活性(PRA)在不同温度条件下对测定结果的影响。方法90例标本分成三组，第一组采血后4℃低温离心。第二组室温放置2h后4℃低温离心。第三组在4℃冰箱放置2h后4℃低温离心。离心后三组都在30min内上机测定结果。另外，将20例标本分别放入4℃和-20℃冰箱，取0d、1d、2d、3d、7d、14d为时间点，测定结果保持稳定的天数。结果在分组实验中第二组与第一组相比AⅡ结果降低(20.21±10.98)%，PRA结果降低(25.6±9.76)%(P<0.05)，第三组与第一组相比AⅡ结果降低(1.60±0.85)%，PRA结果降低(3.3±1.18)%(P>0.05)，在4℃环境中，AⅡ与PRA前3天结果没有显著性差异，P>0.05。3天到14天有显著性差异，P<0.05。在-20℃环境中AⅡ标本在14天内6个时间点的结果没有显著性差异，P>0.05。 PRA在7天内的结果无统计学差异，P>0.05。而7天到14天有显著性差异，P<0.05。结论 AⅡ和PRA对温度敏感，室温放置能使其结果偏低，而在低温环境中能够保持结果稳定。建议AⅡ和PRA标本的运送与预处理都应该在低温中进行，并制定详细的操作规程确保在日常工作中流程的可靠。
目的：研究血管緊張素Ⅱ(AⅡ)和腎素活性(PRA)在不同溫度條件下對測定結果的影響。方法90例標本分成三組，第一組採血後4℃低溫離心。第二組室溫放置2h後4℃低溫離心。第三組在4℃冰箱放置2h後4℃低溫離心。離心後三組都在30min內上機測定結果。另外，將20例標本分彆放入4℃和-20℃冰箱，取0d、1d、2d、3d、7d、14d為時間點，測定結果保持穩定的天數。結果在分組實驗中第二組與第一組相比AⅡ結果降低(20.21±10.98)%，PRA結果降低(25.6±9.76)%(P<0.05)，第三組與第一組相比AⅡ結果降低(1.60±0.85)%，PRA結果降低(3.3±1.18)%(P>0.05)，在4℃環境中，AⅡ與PRA前3天結果沒有顯著性差異，P>0.05。3天到14天有顯著性差異，P<0.05。在-20℃環境中AⅡ標本在14天內6箇時間點的結果沒有顯著性差異，P>0.05。 PRA在7天內的結果無統計學差異，P>0.05。而7天到14天有顯著性差異，P<0.05。結論 AⅡ和PRA對溫度敏感，室溫放置能使其結果偏低，而在低溫環境中能夠保持結果穩定。建議AⅡ和PRA標本的運送與預處理都應該在低溫中進行，併製定詳細的操作規程確保在日常工作中流程的可靠。
목적：연구혈관긴장소Ⅱ(AⅡ)화신소활성(PRA)재불동온도조건하대측정결과적영향。방법90례표본분성삼조，제일조채혈후4℃저온리심。제이조실온방치2h후4℃저온리심。제삼조재4℃빙상방치2h후4℃저온리심。리심후삼조도재30min내상궤측정결과。령외，장20례표본분별방입4℃화-20℃빙상，취0d、1d、2d、3d、7d、14d위시간점，측정결과보지은정적천수。결과재분조실험중제이조여제일조상비AⅡ결과강저(20.21±10.98)%，PRA결과강저(25.6±9.76)%(P<0.05)，제삼조여제일조상비AⅡ결과강저(1.60±0.85)%，PRA결과강저(3.3±1.18)%(P>0.05)，재4℃배경중，AⅡ여PRA전3천결과몰유현저성차이，P>0.05。3천도14천유현저성차이，P<0.05。재-20℃배경중AⅡ표본재14천내6개시간점적결과몰유현저성차이，P>0.05。 PRA재7천내적결과무통계학차이，P>0.05。이7천도14천유현저성차이，P<0.05。결론 AⅡ화PRA대온도민감，실온방치능사기결과편저，이재저온배경중능구보지결과은정。건의AⅡ화PRA표본적운송여예처리도응해재저온중진행，병제정상세적조작규정학보재일상공작중류정적가고。
Objective To study the concentration changes of plasma angiotensinⅡ(AⅡ) and renin activity (PRA) in different temperature conditions. Methods Ninety blood samples were collected from 30 patients and divided into 3 groups. In the first group, the procedure followed the handling protocol provided by the manufacturers for the AⅡand PRA kits precisely. The other two groups kept at room temperature and 40C for 2 hours respectively, and then processed samples by the handling protocol the first group followed. In addition, other 20 specimens were separately stored for 1, 2, 3, 7 and 14 days at 4°C and -20°C to evalu-ate the stability of AⅡand PRA. Results When blood samples were kept at room temperature for 2 hours, the PRA and AⅡde-creased significantly (P<0.05), but both unchanged if samples were placed at 4°C for the same time period. During storage at 4°C, the PRA and AⅡgot no significant changes for 3 days. After 3 days, PRA and AⅡdecreased rapidly. When put into the-20°C en-vironment, the PRA and AⅡwere stabilized for a week. Conclusion The AⅡand PRA were sensitive to temperature, and de-creased quickly in a room temperature. But a low temperature storage condition would remain them in a stable state. So it is neces-sary to apply well-defined pre-analytical procedures for AⅡand PRA measurements.
