CAJ | 학술논문

目的探讨T细胞受体保守域α链基因(TCRCα-575 A/G)多态性与过敏性紫癜(HSP)发病的关系.方法应用聚合酶链反应-限制内切酶片段长度多态分析(PCR-PFLP)方法结合琼脂糖凝胶电泳技术,对36例过敏性紫癜患儿和60例正常对照组儿童进行TCRCα-575 A/G基因多态性分析.结果紫癜组TCRCα基因-575位点的AA、AG、GG基因型频率(22.2％、47.2％、30.6％)与正常组(28.3％、50.0％、21.7％)比较差异无统计学意义(P＞0.05),该位点的等位基因频率A、G(45.8％,54.2％)与正常组(53.3％、46.7％)比较差异无统计学意义(P＞0.05).结论 TCRCα-575 A/G基因多态性变化与HSP的发生无明显关联性.
목적 탐토T세포수체보수역α련기인(TCRCα-575 A/G)다태성여과민성자전(HSP)발병적관계.방법 응용취합매련반응-한제내절매편단장도다태분석(PCR-PFLP)방법결합경지당응효전영기술,대36례과민성자전환인화60례정상대조조인동진행TCRCα-575 A/G기인다태성분석.결과 자전조TCRCα기인-575위점적AA、AG、GG기인형빈솔(22.2％、47.2％、30.6％)여정상조(28.3％、50.0％、21.7％)비교차이무통계학의의(P＞0.05),해위점적등위기인빈솔A、G(45.8％,54.2％)여정상조(53.3％、46.7％)비교차이무통계학의의(P＞0.05).결론 TCRCα-575 A/G기인다태성변화여HSP적발생무명현관련성.
Objective To investigate the genetic polymorphisms of the T cell receptor constant alpha chain gene (TCRCα) loci-575 A/G in Hunan Han Chinese children with Henoch-Schonlein purpura (HSP).Methods Thirty-six cases of patients with HSP and 60 healthy children as controls were investigated in this study.The T cell receptor constant alpha chain gene (TCRCα)loci-575 A/G was detected with polymerase chain reaction-restriction fragment length polymorphism method (PCR-RFLP).Results The genotype frequencies of AA,AG,and GG in the locus-575 A/G of TCRCα gene were 22.2％,47.2％,and 30.6％ in HSP patients,and 28.3％,50.0％,and 21.7％ in control group; and no significant differences were found between HSP patient and control groups (P ＞ 0.05).The allele frequencies of A and G were 45.8％ and 54.2％ in HSP patients,and 53.3％ and 46.7％ in control group; and no significant differences were found between HSP patient and control groups (P ＞ 0.05).Conclusions The polymorphism of locus-575 A/G of T cell receptor constant alpha chain gene had not been found association with HSP.