CAJ | 학술논문

采用免疫亲和层析柱吸附食品样品中的黄曲霉毒素B1，以荧光色谱-柱后光化学衍生法测定食品中黄曲霉毒素B1的含量。样品用甲醇-水溶液(体积比为7∶3)混合，均质器高速搅拌提取，经免疫亲和层析柱纯化后，通过Lachrom C18色谱柱进行分离，以甲醇-水溶液(体积比为45∶55)作为流动相，荧光检测器激发波长为360 nm、发射波长为420 nm，外标法定量。黄曲霉毒素B1的质量浓度在1～20 ng/mL范围内与色谱峰面积线性关系良好，相关系数r=0.9998，检出限为0.1μg/kg。在3个添加水平下，样品的加标回收率在88.5%～97.9%之间，相对标准偏差均小于3%(n=6)。该方法操作简单、定量准确，可用于食品中黄曲霉毒素B1的定量定性检验。
채용면역친화층석주흡부식품양품중적황곡매독소B1，이형광색보-주후광화학연생법측정식품중황곡매독소B1적함량。양품용갑순-수용액(체적비위7∶3)혼합，균질기고속교반제취，경면역친화층석주순화후，통과Lachrom C18색보주진행분리，이갑순-수용액(체적비위45∶55)작위류동상，형광검측기격발파장위360 nm、발사파장위420 nm，외표법정량。황곡매독소B1적질량농도재1～20 ng/mL범위내여색보봉면적선성관계량호，상관계수r=0.9998，검출한위0.1μg/kg。재3개첨가수평하，양품적가표회수솔재88.5%～97.9%지간，상대표준편차균소우3%(n=6)。해방법조작간단、정량준학，가용우식품중황곡매독소B1적정량정성검험。
Aflatoxin B1 in food sample was adsorbed by using immunoaffinity chromatography column, and the content was determined by fluorescence chromatography-post-column photochemical derivatization method. Samples was mixed with methanol-water(Volume ratio was 7∶3),extracted by homogenizer with high speed stirring,purified by im-munoaffinity chromatography column,and separated by Lachrom C18 column with methanol-water (Volume ratio was 45∶55)as the mobile phase and fluorescence detector excitation wavelength of 360 nm,emission wavelength of 420 nm, and determined by external standard method. 1-20 ng/mL aflatoxin B1 content and chromatography peak area had a good linear correlation coefficient(r=0.999 8),the detection limit was 0.1μg/kg. In three spiked levels,spiked recoveries were 88.5%-97.9%,the relative standard deviations were less than 3%(n=6). The improved method is simple,accurate and can be used in food aflatoxin B1 of quantitative and qualitative test.