CAJ | 학술논문

从鱼油中分离制备得到二十碳五烯酸(EPA)甲酯和二十二碳六烯酸(DHA)甲酯，采用红外光谱、质谱、核磁共振波谱等手段对EPA甲酯和DHA甲酯的结构进行表征，提供了不饱和脂肪酸甲酯标准物质研制基础。样品的红外光谱(IR)、质谱(MS)、核磁共振谱图(1H NMR、13C NMR)所给出的结构信息与EPA甲酯和DHA甲酯的化学结构式相符，并通过DEPT谱和13C-1H COSY化学位移相关谱(HMBC)对各共振峰进行了指认，样品的谱图数据与文献报道基本一致。
종어유중분리제비득도이십탄오희산(EPA)갑지화이십이탄륙희산(DHA)갑지，채용홍외광보、질보、핵자공진파보등수단대EPA갑지화DHA갑지적결구진행표정，제공료불포화지방산갑지표준물질연제기출。양품적홍외광보(IR)、질보(MS)、핵자공진보도(1H NMR、13C NMR)소급출적결구신식여EPA갑지화DHA갑지적화학결구식상부，병통과DEPT보화13C-1H COSY화학위이상관보(HMBC)대각공진봉진행료지인，양품적보도수거여문헌보도기본일치。
Eicosapentaenoic acid(EPA) methyl ester and docosahexaenoic acid(DHA) methyl ester were isolated from fish oil, and the unsaturated fatty acid methyl ester standard material development foundation was provided. The structure of EPA methyl ester and DHA methyl ester were characterized by infra-red spectrum(IR),mass spectrometry(MS) and nuclear magnetic resonance spectroscopy(NMR). The structure information given by IR、MS and NMR (1H NMR,13C NMR) of the samples were agreement with the chemical structure formula of EPA methyl ester and DHA methyl ester. In addition,the assignment was studied according to distortionless enhancement by polarization transfer(DEPT) and 13C-1H COSY chemical shift correlation(HMBC),the spectral data of the samples were agree with the literature.