CAJ | 학술논문

目的：研究中药坤宝丸治疗去卵巢小鼠睡眠障碍的实验疗效。方法：选用健康KM小鼠60只，按照体重随机均衡的原则分为4组：假手术组、模型组、地西泮组、坤宝丸组，每组15只。除假手术组外，其余各组均行双侧去卵巢手术，假手术组不摘除卵巢，只剪掉卵巢周围的小部分脂肪组织。坤宝丸组小鼠于术后28天灌胃给予坤宝丸1.667g·kg-1，每天1次，连续28天；地西泮组小鼠于测试前1h单次灌胃给药，给药剂量为1.25mg·kg-1。观察坤宝丸对去卵巢小鼠自主活动、戊巴比妥钠诱导的睡眠及子宫系数的影响。结果：与假手术组相比，小鼠摘除双侧卵巢后自主活动次数和站立次数显著增加（P约0.01），地西泮能够显著降低去卵巢小鼠的自主活动次数（P约0.01），同时可减少小鼠的站立次数，但无统计学差异；坤宝丸具有减少去卵巢小鼠自主活动的趋势，但是作用不显著，对小鼠的站立次数无明显影响。地西泮能够显著延长戊巴比妥钠诱导的去卵巢小鼠睡眠时间（P约0.01），坤宝丸能延长戊巴比妥钠诱导的去卵巢小鼠的睡眠时间，缩短睡眠潜伏期，但均无统计学差异。坤宝丸和地西泮对去卵巢小鼠的子宫系数无明显影响。结论：坤宝丸具有改善去势小鼠睡眠障碍的作用，但作用较温和。
목적：연구중약곤보환치료거란소소서수면장애적실험료효。방법：선용건강KM소서60지，안조체중수궤균형적원칙분위4조：가수술조、모형조、지서반조、곤보환조，매조15지。제가수술조외，기여각조균행쌍측거란소수술，가수술조불적제란소，지전도란소주위적소부분지방조직。곤보환조소서우술후28천관위급여곤보환1.667g·kg-1，매천1차，련속28천；지서반조소서우측시전1h단차관위급약，급약제량위1.25mg·kg-1。관찰곤보환대거란소소서자주활동、무파비타납유도적수면급자궁계수적영향。결과：여가수술조상비，소서적제쌍측란소후자주활동차수화참립차수현저증가（P약0.01），지서반능구현저강저거란소소서적자주활동차수（P약0.01），동시가감소소서적참립차수，단무통계학차이；곤보환구유감소거란소소서자주활동적추세，단시작용불현저，대소서적참립차수무명현영향。지서반능구현저연장무파비타납유도적거란소소서수면시간（P약0.01），곤보환능연장무파비타납유도적거란소소서적수면시간，축단수면잠복기，단균무통계학차이。곤보환화지서반대거란소소서적자궁계수무명현영향。결론：곤보환구유개선거세소서수면장애적작용，단작용교온화。
This article was aimed to study the therapeutic effect of Chinese medicine Kun-Bao-W an (KBW) on sleep disorders among ovariectomized mice. A total of 60 female KM of adult mice were randomly divided into four groups, which were the sham-operated group, model group, diazepam group, and KBW group, with 15 rats in each group. Rats in the sham-operated group were only removed small amount of fatty tissue around the ovaries. Bilateral oophorectomy was given on mice in other groups. In the KBW group, 28 days after the operation, KBW was intragastrically administered (1.667 g·kg-1) every day for 28 days. Mice in the diazepam group were intragas-trically administered (1.25 g·kg-1) 1 h before testing. The observation was made on effects of KBW on locomotor activity, sleeping time of mice induced by pentobarbital sodium and the organ coefficients of uterus. The results showed that compared with sham-operated group, locomotor activity and rearing behavior increased obviously in the model group (P < 0.01). The diazepam group can significantly reduce locomotor activity in ovariectomized mice (P < 0.01), and decrease the number of rearing behavior mildly with no statistical difference. KBW can reduce lo-comotor activity mildly but without effect on rearing behavior in ovariectomized mice. Diazepam can markedly pro-long the pentobarbital sleep time in ovariectomized mice (P < 0.01). KBW can prolong the pentobarbital sleep time and shorten the process of falling into sleep mildly with no statistical difference. There was no significant ef-fect on organ coefficients of uterus in ovariectomized mice by KBW or diazepam. It was concluded that KBW had mild effect on improving sleep disorders in ovariectomized mice.