CAJ | 학술논문

目的：确定慢性阻塞性肺疾病合并肺间质纤维化（PIF-COPD）的动物模型及检测指标。方法：大鼠气管滴入博来霉素（BLM）叠加香烟烟雾刺激构建PIF-COPD动物模型。气管滴入6 mg·kg-1 BLM并香烟烟雾刺激47天，检测肺功能，计算肺指数，HE染色观察肺组织病理形态学改变，天狼星红染色观察胶原I和III变化，用抗体微阵列蛋白质芯片检测血清细胞因子表达谱。结果：呼吸功能检测表明，与对照组相比，模型组大鼠的中心气道阻力、组织阻尼、动态弹性显著升高而动态顺应性显著降低，模型动物肺指数显著增加（P<0.01）；病理检测显示：模型动物肺组织表现为炎性浸润，I、III型胶原含量明显升高，出现肺气肿、纤维化病理表现。模型组大鼠血清MMP-8、TIMP-1、IL-10、IL-13、PDGF-AA和Beta-NGF较正常组增加明显，RAGE较正常组减少。结论：大鼠6 mg·kg-1 BLM气管滴入合并香烟烟雾刺激47天，可作为PIF-COPD的动物模型；模型组大鼠呼吸功能的检测、血清MMP-8、TIMP-1、IL-13、PDGF和NGF可作为该模型的评价指标。
목적：학정만성조새성폐질병합병폐간질섬유화（PIF-COPD）적동물모형급검측지표。방법：대서기관적입박래매소（BLM）첩가향연연무자격구건PIF-COPD동물모형。기관적입6 mg·kg-1 BLM병향연연무자격47천，검측폐공능，계산폐지수，HE염색관찰폐조직병리형태학개변，천랑성홍염색관찰효원I화III변화，용항체미진렬단백질심편검측혈청세포인자표체보。결과：호흡공능검측표명，여대조조상비，모형조대서적중심기도조력、조직조니、동태탄성현저승고이동태순응성현저강저，모형동물폐지수현저증가（P<0.01）；병리검측현시：모형동물폐조직표현위염성침윤，I、III형효원함량명현승고，출현폐기종、섬유화병리표현。모형조대서혈청MMP-8、TIMP-1、IL-10、IL-13、PDGF-AA화Beta-NGF교정상조증가명현，RAGE교정상조감소。결론：대서6 mg·kg-1 BLM기관적입합병향연연무자격47천，가작위PIF-COPD적동물모형；모형조대서호흡공능적검측、혈청MMP-8、TIMP-1、IL-13、PDGF화NGF가작위해모형적평개지표。
This study was aimed to confirm animal model and evaluating indicators of the chronic obstructive pulmonary disease combined with pulmonary interstitial fibrosis (PIF-COPD). PIF-COPD rats were induced by intratracheal administration of Bleomycin(BLM, 6 mg·kg-1) and cigarette smoke stimulation for 47 days. After that, the lung function and index were measured. Changes of pathomorphism of lung were observed with hematoxylin-eosin staining. Collagen protein I and III were determined by Sirius red staining. The protein antibody microarray chip was employed for serum cytokine expression pattern measurement. The results showed that in the lung function testing, the central airway resistance, tissue damping, dynamic elasticity of model rats increased significantly and the dynamic compliance reduced significantly. The lung index of model rats increased significantly (compared with the control group, P < 0.01). The pathological tests showed that lung tissues of model rats showed emphysema and fibration with inflammatory infiltration, and content of collagen type I and III increasing significantly. The contents of MMP-8, TIMP-1, IL-10, IL-13, PDGF-AA, Beta-NGF of model rats serum increased obviously compared with the control group and RAGE reduced compared with the normal control. It was concluded that rats which were induced by intratracheal administration of BLM (6 mg·kg-1) and cigarette smoke stimulation for 47 days can be used as animal model establishment of PIF-COPD. The respiratory function and serum levels of MMP-8, TIMP-1, IL-13, PDGF and NGF can be used as evaluating indicators of PIF-COPD model.