世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2013年
8期
1695-1699
,共5页
方芳%张硕峰%贾占红%张秋霞%王君%段娟娟%邹桂林%何爱先%孙建宁
方芳%張碩峰%賈佔紅%張鞦霞%王君%段娟娟%鄒桂林%何愛先%孫建寧
방방%장석봉%가점홍%장추하%왕군%단연연%추계림%하애선%손건저
杞菊润目贴%干眼症%盐酸平阳霉素%大鼠
杞菊潤目貼%榦眼癥%鹽痠平暘黴素%大鼠
기국윤목첩%간안증%염산평양매소%대서
Qiju Runmu Tie%dry eye syndrome%bleomycin hydrochloride%rat
目的：观察杞菊润目贴对实验性大鼠干眼症的改善作用。方法：将盐酸平阳霉素注射液（8 mg·mL-1）注入大鼠框内（0.06 mL）和框外泪腺（0.12 mL）后第4天，杞菊润目贴大、中、小剂量（分别为0.276、0.138、0.069 g·mL-1）组大鼠连续敷眼10天，每天1次，每次1 h，假手术组和模型组大鼠按同样操作，敷上含蒸馏水的贴剂。第11天~13天敷眼和滴眼后进行泪液分泌量、泪膜破裂时间及泪腺组织结构病理改变测定。结果：盐酸平阳霉素可引起大鼠泪腺组织萎缩，增生及腺泡腔内泡状粘液减少等损伤；与假手术组比较，模型组大鼠泪液分泌量显著减少（P<0.01）；泪膜破裂时间显著缩短（P<0.01）。与模型组比较，杞菊润目贴各剂量均可显著增加泪液分泌量（P<0.05），延长泪膜破裂时间（P<0.01），杞菊润目贴中剂量减轻泪腺组织损伤程度的作用明显。结论：杞菊润目贴外用对实验性大鼠干眼症有改善作用。
目的：觀察杞菊潤目貼對實驗性大鼠榦眼癥的改善作用。方法：將鹽痠平暘黴素註射液（8 mg·mL-1）註入大鼠框內（0.06 mL）和框外淚腺（0.12 mL）後第4天，杞菊潤目貼大、中、小劑量（分彆為0.276、0.138、0.069 g·mL-1）組大鼠連續敷眼10天，每天1次，每次1 h，假手術組和模型組大鼠按同樣操作，敷上含蒸餾水的貼劑。第11天~13天敷眼和滴眼後進行淚液分泌量、淚膜破裂時間及淚腺組織結構病理改變測定。結果：鹽痠平暘黴素可引起大鼠淚腺組織萎縮，增生及腺泡腔內泡狀粘液減少等損傷；與假手術組比較，模型組大鼠淚液分泌量顯著減少（P<0.01）；淚膜破裂時間顯著縮短（P<0.01）。與模型組比較，杞菊潤目貼各劑量均可顯著增加淚液分泌量（P<0.05），延長淚膜破裂時間（P<0.01），杞菊潤目貼中劑量減輕淚腺組織損傷程度的作用明顯。結論：杞菊潤目貼外用對實驗性大鼠榦眼癥有改善作用。
목적：관찰기국윤목첩대실험성대서간안증적개선작용。방법：장염산평양매소주사액（8 mg·mL-1）주입대서광내（0.06 mL）화광외루선（0.12 mL）후제4천，기국윤목첩대、중、소제량（분별위0.276、0.138、0.069 g·mL-1）조대서련속부안10천，매천1차，매차1 h，가수술조화모형조대서안동양조작，부상함증류수적첩제。제11천~13천부안화적안후진행루액분비량、루막파렬시간급루선조직결구병리개변측정。결과：염산평양매소가인기대서루선조직위축，증생급선포강내포상점액감소등손상；여가수술조비교，모형조대서루액분비량현저감소（P<0.01）；루막파렬시간현저축단（P<0.01）。여모형조비교，기국윤목첩각제량균가현저증가루액분비량（P<0.05），연장루막파렬시간（P<0.01），기국윤목첩중제량감경루선조직손상정도적작용명현。결론：기국윤목첩외용대실험성대서간안증유개선작용。
This study was aimed to observe the improvement of Qiju Runmu Tie (QRT) on experimental dry eye syndrome in rats. The fourth day after the bleomycin hydrochloride (8 g·mL-1) was injected into the intraorbital lacrimal gland (0.06 mL) and extraorbital lacrimal gland (0.12 mL), QRT of large, medium, and small dosage (0.276 g·mL-1, 0.138 g·mL-1, 0.069 g·mL-1) was applied on rat's eyes continuously for 10 days, once a day, 1 hour every time. Rats in the control group and model group were applied with a patch containing distilled water in the same operation. The tear secretions, time of tear film break-up and pathological changes of lacrimal gland were observed from the eleventh day to the thirteenth day after injection. The results showed that compared with the control group, bleomycin hydrochloride can induce atrophy of lacrimal gland, hyperplasia and decrease bubbly mucus in the acinar lumina, significant reduce of tear secretion, and significant shorten the time of tear film break-up. Compared with the model group, QRT can significantly increase the amount of tear secretion (P <0.05), extend the tear film break-up time (P < 0.01). And the medium dosage of QRT can obviously reduce lacrimal gland tissue injury. It was concluded that external application of QRT can improve symptoms of experi-mental dry eye syndrome in rats.
