CAJ | 학술논문

目的：探讨梓醇对永久性脑缺血模型急性期脑组织梗死体积、含水量及亚急性期炎性反应的影响。方法：采用化学刺激法建立局灶性脑缺血（MCAT）模型，检测急性期（24 h）神经行为学症状、脑梗死面积和脑含水量；线栓法制作永久性大鼠脑缺血（pMCAO）模型，酶联免疫吸附法（ELISA）测定缺血侧脑组织白细胞介素-6（IL-6）、白细胞介素-10（IL-10）和核转录因子Bp65（NF-κBp65）的含量。结果：大鼠MCAT后24 h，梓醇15~60 mg·kg-1剂量组可以显著改善模型动物神经症状损伤（P<0.01或P<0.001），梓醇15 mg·kg-1组可显著降低模型动物梗塞区面积（P<0.05），30 mg·kg-1组和60 mg·kg-1组可显著降低脑水肿（P<0.05）；大鼠pMCAO术后7天开始，梓醇30 mg·kg-1组或60 mg·kg-1组开始改善模型动物神经症状损伤；术后14天，与假手术组比较，缺血侧脑组织IL-10和核转录因子NF-κBp65的含量变化与模型组已经不明显，IL-6水平显著降低（P<0.05），梓醇15 mg·kg-1灌胃14天可以降低模型动物缺血侧脑组织NF-κBp65含量（P<0.05）。结论：梓醇能改善局灶性脑缺血模型动物急性期及亚急性期神经症状损伤，缩小梗死灶，减轻脑部水肿，其作用可能与抑制脑缺血引起的炎症损伤无关。
목적：탐토재순대영구성뇌결혈모형급성기뇌조직경사체적、함수량급아급성기염성반응적영향。방법：채용화학자격법건립국조성뇌결혈（MCAT）모형，검측급성기（24 h）신경행위학증상、뇌경사면적화뇌함수량；선전법제작영구성대서뇌결혈（pMCAO）모형，매련면역흡부법（ELISA）측정결혈측뇌조직백세포개소-6（IL-6）、백세포개소-10（IL-10）화핵전록인자Bp65（NF-κBp65）적함량。결과：대서MCAT후24 h，재순15~60 mg·kg-1제량조가이현저개선모형동물신경증상손상（P<0.01혹P<0.001），재순15 mg·kg-1조가현저강저모형동물경새구면적（P<0.05），30 mg·kg-1조화60 mg·kg-1조가현저강저뇌수종（P<0.05）；대서pMCAO술후7천개시，재순30 mg·kg-1조혹60 mg·kg-1조개시개선모형동물신경증상손상；술후14천，여가수술조비교，결혈측뇌조직IL-10화핵전록인자NF-κBp65적함량변화여모형조이경불명현，IL-6수평현저강저（P<0.05），재순15 mg·kg-1관위14천가이강저모형동물결혈측뇌조직NF-κBp65함량（P<0.05）。결론：재순능개선국조성뇌결혈모형동물급성기급아급성기신경증상손상，축소경사조，감경뇌부수종，기작용가능여억제뇌결혈인기적염증손상무관。
This study was aimed to explore the effect of Catalpol on the cerebral infarction size in acute phase, water content and inflammatory reaction of early recovery after permanent middle cerebral artery occlusion (pM-CAO). Adult male Sprague-dawley rats were subjected to chemical method to establish MCAT model. The detec-tion was made on neurobehavioral symptoms, cerebral infarction volume and water content at 24 h after surgery. The content of intedeukin-6 (IL-6), intedeukin-10 (IL-10) and nuclear factor kappa Bp65 (NF-κBp65) were de-tected after pMCAO with enzyme-linked immunosorbent assay (ELISA). The results showed that 24 h after MCAT, Catalpol 15-60 mg·kg-1 can significantly improve the neurobehavioral symptoms (P < 0.01, or P <0.001). The Catalpol 15 mg·kg-1 can significantly reduce cerebral infarction volume (P < 0.05). The Catalpol 30-60 mg·kg-1 can significantly reduce water content (P < 0.05). Catalpol 30-60 mg·kg-1 can significantly improve neurobehav-ioral symptoms from the 7th day after pMCAO. On the 14th after pMCAO, the content of IL-10 and NF-κBp65 of ischemia brain had no difference compared with sham-operated group. The IL-6 level of ischemia brain was obvi-ously reduced than the sham-operated group(P < 0.05). The intragastric administration of Catalpol 15 mg·kg-1 for 14 days can reduce the content of NF-κBp65 in the ischemia brain of model rats (P < 0.05). It was concluded that Catalpol can improve neurobehavioral symptoms of acute and subacute phase after cerebral ischemia, reduce infarcts and water content. These effects may not be related with its inhibition of inflammatory derived from cere-bral ischemia.