世界科学技术-中医药现代化
世界科學技術-中醫藥現代化
세계과학기술-중의약현대화
WORLD SCIENCE AND TECHNOLOGY-MODERNIZATION OF TRADITIONAL CHINESE MEDICINE
2013年
8期
1676-1681
,共6页
张胜威%董世芬%武汀%孙建宁
張勝威%董世芬%武汀%孫建寧
장성위%동세분%무정%손건저
梓醇%实时步态行为%缺血性卒中%pMCAO大鼠
梓醇%實時步態行為%缺血性卒中%pMCAO大鼠
재순%실시보태행위%결혈성졸중%pMCAO대서
Catalpol%real-time gait behavior%cerebral ischemia%pMCAO rat
目的：观察梓醇对pMCAO大鼠恢复期早期实时步态的影响，评价其对缺血性卒中的改善作用。方法：将大鼠随机分为6组，即正常对照组，模型组，阳性药天保宁组，梓醇15、30、60 mg·kg-1组。于术前进行为期7天的步态仪行走训练，每3次。训练完成后，采用线栓法建立永久性大脑中动脉栓塞（pM原CAO）大鼠模型，并于术后3~14天连续给药，术后第14天进行实时步态行为检测。结果：术后14天，与正常组比较，pMCAO模型大鼠平均步行周期显著延长（P<0.01），绝对值平均体转角明显升高（P<0.05），双足支撑时长缩短（P<0.05），三足支撑时长延长（P<0.01），协调性指标显著增加（P<0.01）。与模型组比较，梓醇30 mg·kg-1及60 mg·kg-1组模型动物步行周期、降低绝对值平均体转角显著缩短（P<0.05或P<0.01）；梓醇30 mg·kg-1组右前相对于其他三足的协调性指数明显降低（P<0.05），脑缺血动物协调性提高。结论：梓醇可部分改善脑缺血模型大鼠的实时步态行为变化，对缺血性卒中的神经功能有一定保护作用。
目的：觀察梓醇對pMCAO大鼠恢複期早期實時步態的影響，評價其對缺血性卒中的改善作用。方法：將大鼠隨機分為6組，即正常對照組，模型組，暘性藥天保寧組，梓醇15、30、60 mg·kg-1組。于術前進行為期7天的步態儀行走訓練，每3次。訓練完成後，採用線栓法建立永久性大腦中動脈栓塞（pM原CAO）大鼠模型，併于術後3~14天連續給藥，術後第14天進行實時步態行為檢測。結果：術後14天，與正常組比較，pMCAO模型大鼠平均步行週期顯著延長（P<0.01），絕對值平均體轉角明顯升高（P<0.05），雙足支撐時長縮短（P<0.05），三足支撐時長延長（P<0.01），協調性指標顯著增加（P<0.01）。與模型組比較，梓醇30 mg·kg-1及60 mg·kg-1組模型動物步行週期、降低絕對值平均體轉角顯著縮短（P<0.05或P<0.01）；梓醇30 mg·kg-1組右前相對于其他三足的協調性指數明顯降低（P<0.05），腦缺血動物協調性提高。結論：梓醇可部分改善腦缺血模型大鼠的實時步態行為變化，對缺血性卒中的神經功能有一定保護作用。
목적：관찰재순대pMCAO대서회복기조기실시보태적영향，평개기대결혈성졸중적개선작용。방법：장대서수궤분위6조，즉정상대조조，모형조，양성약천보저조，재순15、30、60 mg·kg-1조。우술전진행위기7천적보태의행주훈련，매3차。훈련완성후，채용선전법건립영구성대뇌중동맥전새（pM원CAO）대서모형，병우술후3~14천련속급약，술후제14천진행실시보태행위검측。결과：술후14천，여정상조비교，pMCAO모형대서평균보행주기현저연장（P<0.01），절대치평균체전각명현승고（P<0.05），쌍족지탱시장축단（P<0.05），삼족지탱시장연장（P<0.01），협조성지표현저증가（P<0.01）。여모형조비교，재순30 mg·kg-1급60 mg·kg-1조모형동물보행주기、강저절대치평균체전각현저축단（P<0.05혹P<0.01）；재순30 mg·kg-1조우전상대우기타삼족적협조성지수명현강저（P<0.05），뇌결혈동물협조성제고。결론：재순가부분개선뇌결혈모형대서적실시보태행위변화，대결혈성졸중적신경공능유일정보호작용。
This study was aimed to observe the influence of Catalpol on real-time gait analysis of early recovery after permanent middle cerebral artery occlusion (pMCAO) in rats to evaluate its effect on the improvement after cerebral ischemia. Rats were randomly divided into 6 groups, which were the normal control group, model group, Tianbaoning group, Catalpol 15, 30, 60 mg·kg-1 group. Rats were trained on the gait instrument for 7 days before pMCAO, 3 times/day. After the training, pMCAO model was made. And continuous infusion was performed from the 3rd to the 14th day after the operation. Then, the real-time gait behavior was detected on the 14th day. The re-sults showed that 14 days after the surgery, compared with the normal control group, the models had a significant extending in the duty cycle (P < 0.01), and obvious increasing of the average body angle of absolute value (P <0.05), the shortening of the two feet supporting time (P < 0.05), and extending of three feet supporting time (P <0.01), and increasing of the coordination index (P < 0.01). Compared with the model group, the Catalpol of 30 mg·kg-1 and 60 mg·kg-1 group has obviously decreased duty cycle and average body angle of absolute value (P <0.05, or P < 0.01). The Catalpol of 30 mg·kg-1 can obviously reduce the coordination index of the right front foot relative to the other three feet (P < 0.05), which improved the coordination of cerebral ischemia animals. It was concluded that Catalpol can improve the real-time gait behavior changes of cerebral ischemia model rats. There-fore, Catalpol have a neural protective effect on cerebral ischemia.
