中国医学影像学杂志
中國醫學影像學雜誌
중국의학영상학잡지
CHINESE JOURNAL OF MEDICAL IMAGING
2013年
10期
729-732,736
,共5页
郝兰%赵雅静%王志刚%冉海涛%宋卫香
郝蘭%趙雅靜%王誌剛%冉海濤%宋衛香
학란%조아정%왕지강%염해도%송위향
卵巢肿瘤%肿瘤细胞,培养的%超声疗法%量子点%微气泡%光化学疗法%细胞增殖%细胞凋亡%体外研究
卵巢腫瘤%腫瘤細胞,培養的%超聲療法%量子點%微氣泡%光化學療法%細胞增殖%細胞凋亡%體外研究
란소종류%종류세포,배양적%초성요법%양자점%미기포%광화학요법%세포증식%세포조망%체외연구
Ovarian neoplasms%Tumor cells,cultured%Ultrasonic therapy%Quantum dots%Microbubbles%Photochemotherapy%Cell proliferation%Apoptosis%In vitro
目的:以自制载量子点(QDs)乳酸/羟基乙酸共聚物(PLGA)微泡(MBQDs/PLGA)为光敏剂,超声(US)联合光动力疗法(PDT)处理人卵巢癌SKOV3细胞,研究细胞凋亡机制。材料与方法以双乳化法制备MBQDs/PLGA,采用MTT法比较QDs与MBQDs/PLGA的细胞毒活性,确定处理条件。在选择条件下处理SKOV3,行HE染色观察细胞受损情况,以流式细胞仪双染检测细胞凋亡情况。结果以PDT能量密度为180.0 J/cm2、US声强为0.5 W/cm2(1.0 MHz,10 s)处理细胞,US-PDT-MBQDs/PLGA和PDT-MBQDs/PLGA两组显微镜下可见细胞变形,细胞间失去彼此连接;透射电镜下可见致密的染色质沿核膜下聚集,有凋亡小体形成;最大细胞凋亡率为(22.17±0.38)%。结论 MBQDs/PLGA介导的PDT对SKOV3细胞具有增殖抑制作用,且低功率超声辐照因声孔效应能协同增效MBQDs/PLGA的PDT作用。
目的:以自製載量子點(QDs)乳痠/羥基乙痠共聚物(PLGA)微泡(MBQDs/PLGA)為光敏劑,超聲(US)聯閤光動力療法(PDT)處理人卵巢癌SKOV3細胞,研究細胞凋亡機製。材料與方法以雙乳化法製備MBQDs/PLGA,採用MTT法比較QDs與MBQDs/PLGA的細胞毒活性,確定處理條件。在選擇條件下處理SKOV3,行HE染色觀察細胞受損情況,以流式細胞儀雙染檢測細胞凋亡情況。結果以PDT能量密度為180.0 J/cm2、US聲彊為0.5 W/cm2(1.0 MHz,10 s)處理細胞,US-PDT-MBQDs/PLGA和PDT-MBQDs/PLGA兩組顯微鏡下可見細胞變形,細胞間失去彼此連接;透射電鏡下可見緻密的染色質沿覈膜下聚集,有凋亡小體形成;最大細胞凋亡率為(22.17±0.38)%。結論 MBQDs/PLGA介導的PDT對SKOV3細胞具有增殖抑製作用,且低功率超聲輻照因聲孔效應能協同增效MBQDs/PLGA的PDT作用。
목적:이자제재양자점(QDs)유산/간기을산공취물(PLGA)미포(MBQDs/PLGA)위광민제,초성(US)연합광동력요법(PDT)처리인란소암SKOV3세포,연구세포조망궤제。재료여방법이쌍유화법제비MBQDs/PLGA,채용MTT법비교QDs여MBQDs/PLGA적세포독활성,학정처리조건。재선택조건하처리SKOV3,행HE염색관찰세포수손정황,이류식세포의쌍염검측세포조망정황。결과이PDT능량밀도위180.0 J/cm2、US성강위0.5 W/cm2(1.0 MHz,10 s)처리세포,US-PDT-MBQDs/PLGA화PDT-MBQDs/PLGA량조현미경하가견세포변형,세포간실거피차련접;투사전경하가견치밀적염색질연핵막하취집,유조망소체형성;최대세포조망솔위(22.17±0.38)%。결론 MBQDs/PLGA개도적PDT대SKOV3세포구유증식억제작용,차저공솔초성복조인성공효응능협동증효MBQDs/PLGA적PDT작용。
Purpose To explore the apoptosis mechanism of human ovarian cancer cells SKOV3 processed with ultrasound (US) combined with photodynamic therapy (PDT), with self-contained lactic acid/glycolic acid (PLGA) microbubbles (MBQDs/PLGA) containing quantum dots (QDs) as a photosensitizer. Materials and Methods MBQDs/PLGA was prepared with double emulsion method, and MTT was used to compare the cytotoxic activity difference between QDs and MBQDs/PLGA, to determine the proper treatment condition. SKOV3 cells were treated under selective conditions, cell damage manifestation was observed with HE staining, and double staining flow cytometry was used to detect cell apoptosis. Results When cells were treated with PDT with energy density of 180.0 J/cm2 and US with sound intensity of 0.5 W/cm2 (1.0 MHz, 10 s), cell deformation could be observed under a microscope in both US-PDT-MBQDs/PLGA group and PDT-MBQDs/PLGA group, and cell connections were absent between cells;transmission electron microscope showed dense chromatin gathered along the nuclear membrane, with the formation of apoptotic bodies also be displayed. Maximum apoptosis rate was (22.17±0.38)% Conclusion MBQDs/PLGA-mediated PDT contains proliferation inhibition effect for SKOV3 cells, which can be synergied with low-power ultrasonic irradiation due to sonoporation effect.