CAJ | 학술논문

为了获取到高质量的刺五加总RNA，以叶片为试材，比较不同提取方法的提取效果。结果表明，改良的异硫氰酸胍法效果最优，方法简单、经济，通过该方法获得的总RNA能够很好的满足后续分子生物学实验的要求，试剂盒方法效果次之，CTAB法效果第三，Trizol法最差。
위료획취도고질량적자오가총RNA，이협편위시재，비교불동제취방법적제취효과。결과표명，개량적이류청산고법효과최우，방법간단、경제，통과해방법획득적총RNA능구흔호적만족후속분자생물학실험적요구，시제합방법효과차지，CTAB법효과제삼，Trizol법최차。
In order to get the high quality of total RNA of Eleutherococcus senticosus, the extraction effects of different extraction methods were compared by taking the leaf of E. senticosus as the test materials. The results indicated that the improved guanidine thiocyanate method is the best which is simple and economic. The total RNA extracted with this method can satisfy the requirements of the molecular biology of the follow-up experiments. And it is followed by the kit method. The effect of CTAB method is third, and the Trizol method is the worst.