CAJ | 학술논문

目的：探讨分析不同检测方法在糖化血红蛋白定量中的应用价值，丰富临床检测经验。方法：选择我院于2011年11月-2013年3月在我院接受治疗的糖尿病患者550例，通过全自动生化分析仪检测患者的血红蛋白A1c水平，依据血红蛋白A1c水平将其分成低值组、中值组以及高值组，同时通过离子交换高效液相色谱分析法（HPLC）以及酶法对不同组别的血红蛋白 A1c待检样本进行检测，观察不同检测方法在糖化血红蛋白定量中的应用价值；同时应用酶法和 HPLC 对患者的高脂蛋白进行检测。结果：两种检测方法均具有较高的准确性，但是检测结果显示应用酶法检测糖化血红蛋白的水平略高于应用 HPLC的检测结果，但是两组比较差异不显著（P＞0．05）；检测结果显示酶法检测的高脂蛋白值无异常，而应用 HPLC法检测的高脂蛋白值偏高，两组比较差异显著（P＜0．05）。结论：酶法与 HPLC 在糖化血红蛋白的定量中有较高的应用价值，均具有较高的可信度，但是相比之下酶法检测的操作更为简便、快捷，且较为经济，因此酶法可作为糖化血红蛋白定量的主要检测方法。
목적：탐토분석불동검측방법재당화혈홍단백정량중적응용개치，봉부림상검측경험。방법：선택아원우2011년11월-2013년3월재아원접수치료적당뇨병환자550례，통과전자동생화분석의검측환자적혈홍단백A1c수평，의거혈홍단백A1c수평장기분성저치조、중치조이급고치조，동시통과리자교환고효액상색보분석법（HPLC）이급매법대불동조별적혈홍단백 A1c대검양본진행검측，관찰불동검측방법재당화혈홍단백정량중적응용개치；동시응용매법화 HPLC 대환자적고지단백진행검측。결과：량충검측방법균구유교고적준학성，단시검측결과현시응용매법검측당화혈홍단백적수평략고우응용 HPLC적검측결과，단시량조비교차이불현저（P＞0．05）；검측결과현시매법검측적고지단백치무이상，이응용 HPLC법검측적고지단백치편고，량조비교차이현저（P＜0．05）。결론：매법여 HPLC 재당화혈홍단백적정량중유교고적응용개치，균구유교고적가신도，단시상비지하매법검측적조작경위간편、쾌첩，차교위경제，인차매법가작위당화혈홍단백정량적주요검측방법。
objective :to study the analysis of different testing methods in the application of glycosylated hemoglobin quantitative value , rich clinical experience .Methods :our hospital in November 2011 - March 2013 in our hospital for treatment of 550 patients with diabetes , patients with automatic biochemical analyzer test hemoglobin A1c levels ,on the basis of hemoglobin A1c levels will be divided into low group ,the median and high value group ,at the same time through ion exchange high performance liquid chromatography (HPLC) and enzy-matic for different groups of hemoglobin A1c inspected samples for testing ,observe different testing methods in the application of glycosylat-ed hemoglobin quantitative value ;At the same time the enzymatic and HPLC to detect patients with high cholesterol .Results :the two kinds of detection method has higher accuracy ,but the detection results show that the application of enzymatic saccharification hemoglobin level slightly higher than the application of HPLC test results ,but no significant difference compared the two groups (P > 0 .05);Test results show that the enzymatic test of high lipoprotein values without exception ,and the application of HPLC to detect high lipoprotein values on the high side ,two groups are significant difference (P < 0 .05) .Conclusion :enzymatic and HPLC in the quantitative of glycosylated hemo-globin has higher application value and have higher credibility ,but compared with enzymatic detection operation more simple and convenient , and more economic ,therefore enzymatic can be used as the main detection methods glycated hemoglobin quantitative .