中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2013年
14期
2086-2088
,共3页
荧光素酶法,细菌%外科器械%质量控制
熒光素酶法,細菌%外科器械%質量控製
형광소매법,세균%외과기계%질량공제
Luciferases,bacteria%Surgical instruments%Quality control
目的 探讨三磷酸腺苷(ATP)生物荧光法在监测宫腔吸管清洗质量中的应用价值.方法 按随机原则选择使用后的宫腔吸管120件,A组60件采用纯水浸泡+高压水枪冲洗+干燥的方法,B组60件 采用碱性清洗液浸泡+超声+高压水枪冲洗+手工擦洗+干燥的方法.用目测法初步检测宫腔吸管清洗后的质量,用ATP生物荧光法和细菌计数法作平行比较,检测宫腔吸管头内部顶端、吸管接头端内口2.5 cm处、吸管外壁前端、吸管腔内壁10 cm处,清洗前后细菌的残留量.结果 两组宫腔吸管完成清洗干燥程序后吸管头内部顶端、吸管接头端内口2.5 cm处、吸管外壁前端、吸管腔内壁10 cm处相对光单位值(RLU)差异均有统计学意义(t =26.81、29.13、7.14、55.74,均P＜0.01),B组清洗效果明显优于A组.细菌计数法检测未检出致病微生物,上述四个部位清洗合格率分别是:A组:70.0％、85.0％、93.3％、93.3％；B组:95.0％、98.3％、100.0％、100.0％；B组合格率均高于A组(x2=12.99、6.98、4.14、4.14,均P＜0.05).结论 碱性清洗液浸泡、超声和手工擦洗是宫腔吸管清洗的重要环节；ATP生物荧光法可动态地快速检测宫腔吸管清洗前后的细菌残留量,能作为有效检测宫腔吸管清洗质量的评价方法.
目的 探討三燐痠腺苷(ATP)生物熒光法在鑑測宮腔吸管清洗質量中的應用價值.方法 按隨機原則選擇使用後的宮腔吸管120件,A組60件採用純水浸泡+高壓水鎗遲洗+榦燥的方法,B組60件 採用堿性清洗液浸泡+超聲+高壓水鎗遲洗+手工抆洗+榦燥的方法.用目測法初步檢測宮腔吸管清洗後的質量,用ATP生物熒光法和細菌計數法作平行比較,檢測宮腔吸管頭內部頂耑、吸管接頭耑內口2.5 cm處、吸管外壁前耑、吸管腔內壁10 cm處,清洗前後細菌的殘留量.結果 兩組宮腔吸管完成清洗榦燥程序後吸管頭內部頂耑、吸管接頭耑內口2.5 cm處、吸管外壁前耑、吸管腔內壁10 cm處相對光單位值(RLU)差異均有統計學意義(t =26.81、29.13、7.14、55.74,均P＜0.01),B組清洗效果明顯優于A組.細菌計數法檢測未檢齣緻病微生物,上述四箇部位清洗閤格率分彆是:A組:70.0％、85.0％、93.3％、93.3％；B組:95.0％、98.3％、100.0％、100.0％；B組閤格率均高于A組(x2=12.99、6.98、4.14、4.14,均P＜0.05).結論 堿性清洗液浸泡、超聲和手工抆洗是宮腔吸管清洗的重要環節；ATP生物熒光法可動態地快速檢測宮腔吸管清洗前後的細菌殘留量,能作為有效檢測宮腔吸管清洗質量的評價方法.
목적 탐토삼린산선감(ATP)생물형광법재감측궁강흡관청세질량중적응용개치.방법 안수궤원칙선택사용후적궁강흡관120건,A조60건채용순수침포+고압수창충세+간조적방법,B조60건 채용감성청세액침포+초성+고압수창충세+수공찰세+간조적방법.용목측법초보검측궁강흡관청세후적질량,용ATP생물형광법화세균계수법작평행비교,검측궁강흡관두내부정단、흡관접두단내구2.5 cm처、흡관외벽전단、흡관강내벽10 cm처,청세전후세균적잔류량.결과 량조궁강흡관완성청세간조정서후흡관두내부정단、흡관접두단내구2.5 cm처、흡관외벽전단、흡관강내벽10 cm처상대광단위치(RLU)차이균유통계학의의(t =26.81、29.13、7.14、55.74,균P＜0.01),B조청세효과명현우우A조.세균계수법검측미검출치병미생물,상술사개부위청세합격솔분별시:A조:70.0％、85.0％、93.3％、93.3％；B조:95.0％、98.3％、100.0％、100.0％；B조합격솔균고우A조(x2=12.99、6.98、4.14、4.14,균P＜0.05).결론 감성청세액침포、초성화수공찰세시궁강흡관청세적중요배절；ATP생물형광법가동태지쾌속검측궁강흡관청세전후적세균잔류량,능작위유효검측궁강흡관청세질량적평개방법.
Objective To explore the application value of ATP bioluminescence in assaying the cleaning quality of uterine suction.Methods According to the principle of random,we selected 120 uterine suction tube after use.60 used tubes in A group were given water immersion + high-pressure water jets wash + dry method,60 used tubes in B group were given alkaline cleaning solution soak + ultrasonic + high-pressure water washing + manual scrub + drying method.The quality of suction tube was initially tested with the visual method after cleaning,Straw inside the top cavity,2.5cm at inside straw joint and the front of straw outer wall,10cm at suction cavity wall,before and after cleaning the residues of bacteria were assayed with the ATP bioluminescence and bacteria counting method for parallel comparison.Results After cleaning drying program,the RLU of two groups in suction head internal top,2.5cm at inside straw joint,the front of straw outer wall,10cm at suction cavity wall had significant differences (t =26.81,29.13,7.14,55.74,P ＜ 0.01).The cleaning effect of B group was better than A group..Bacteria counting method was used to detect pathogenic microorganism that was not detected.The qualified rate of cleaning at four parts was as following:group A:70.0％,85.0％,93.3％,93.3％.B group:95.0％,98.3％,100.0％,100.0％,and the qualified rate of group B was significantly higher than group A (x2 =12.99,6.98,4.14,4.14,all P ＜ 0.05).Conclusion ATP bioluminescence method can dynamically rapid detect the bacteria before and after intrauterine suction cleaning residues,can be viewed as an effective method to detect quality of uterine suction cleaning.
