中国动物保健
中國動物保健
중국동물보건
CHINA ANIMAL HEALTH
2013年
12期
16-21,22
,共7页
陈海娟%孙心%李淑兰%李敏%胡瑞红%吴强
陳海娟%孫心%李淑蘭%李敏%鬍瑞紅%吳彊
진해연%손심%리숙란%리민%호서홍%오강
佐剂%重组禽流感%灭活苗
佐劑%重組禽流感%滅活苗
좌제%중조금류감%멸활묘
adjuvant%recombinant avian influenza%inactivated vaccine
禽流感病毒(Avian Influenza Virus, AIV)是禽流行性感冒(简称禽流感, Avian Influenza, AI)的病原。其中高致病性的H5N1亚型AIV可以造成鸡群的大批死亡,对家禽养殖业影响严重,并可引起人的感染和死亡。通过接种疫苗能有效地控制禽流感的传播,专家们认为,选择优质的疫苗佐剂是新型疫苗走向成功的关键。本试验将同一批次的禽流感(H5N1亚型,Re-6株)抗原分别与国产白油佐剂、法国进口白油佐剂及美国进口白油佐剂3种佐剂制备成重组禽流感病毒灭活疫苗(H5N1亚型,Re-6株),进行物理性状、无菌检验,均符合规定标准。将以上三种疫苗分别免疫21日龄SPF鸡,0.3mL/只,在免疫后的一周、两周、三周、四周,每组抽取10只分别采血,分离血清;18日龄蛋鸡0.3mL/只,在免疫后的一周、两周、三周,每组抽取10只分别采血,分离血清,21d后二免0.5mL/只,二免蛋鸡在免疫后的两周、三周、四周、两个月、三个月每组抽取10只分别采血,分离血清;每次连同对照组5只血清用禽流感病毒H5亚型(Re-6株)抗原测定HI抗体,试验结果表明,美国进口白油佐剂制备的重组禽流感病毒灭活疫苗(H5N1亚型, Re-6株)免疫鸡群后,HI抗体效价最高,免疫持续一段时间后,效价仍保持较高,同时做安全检验对比试验,各肌肉注射疫苗2.0mL/只,连续观察14d,试验结果表明,以上三种佐剂制备的疫苗均安全无副反应。
禽流感病毒(Avian Influenza Virus, AIV)是禽流行性感冒(簡稱禽流感, Avian Influenza, AI)的病原。其中高緻病性的H5N1亞型AIV可以造成鷄群的大批死亡,對傢禽養殖業影響嚴重,併可引起人的感染和死亡。通過接種疫苗能有效地控製禽流感的傳播,專傢們認為,選擇優質的疫苗佐劑是新型疫苗走嚮成功的關鍵。本試驗將同一批次的禽流感(H5N1亞型,Re-6株)抗原分彆與國產白油佐劑、法國進口白油佐劑及美國進口白油佐劑3種佐劑製備成重組禽流感病毒滅活疫苗(H5N1亞型,Re-6株),進行物理性狀、無菌檢驗,均符閤規定標準。將以上三種疫苗分彆免疫21日齡SPF鷄,0.3mL/隻,在免疫後的一週、兩週、三週、四週,每組抽取10隻分彆採血,分離血清;18日齡蛋鷄0.3mL/隻,在免疫後的一週、兩週、三週,每組抽取10隻分彆採血,分離血清,21d後二免0.5mL/隻,二免蛋鷄在免疫後的兩週、三週、四週、兩箇月、三箇月每組抽取10隻分彆採血,分離血清;每次連同對照組5隻血清用禽流感病毒H5亞型(Re-6株)抗原測定HI抗體,試驗結果錶明,美國進口白油佐劑製備的重組禽流感病毒滅活疫苗(H5N1亞型, Re-6株)免疫鷄群後,HI抗體效價最高,免疫持續一段時間後,效價仍保持較高,同時做安全檢驗對比試驗,各肌肉註射疫苗2.0mL/隻,連續觀察14d,試驗結果錶明,以上三種佐劑製備的疫苗均安全無副反應。
금류감병독(Avian Influenza Virus, AIV)시금류행성감모(간칭금류감, Avian Influenza, AI)적병원。기중고치병성적H5N1아형AIV가이조성계군적대비사망,대가금양식업영향엄중,병가인기인적감염화사망。통과접충역묘능유효지공제금류감적전파,전가문인위,선택우질적역묘좌제시신형역묘주향성공적관건。본시험장동일비차적금류감(H5N1아형,Re-6주)항원분별여국산백유좌제、법국진구백유좌제급미국진구백유좌제3충좌제제비성중조금류감병독멸활역묘(H5N1아형,Re-6주),진행물이성상、무균검험,균부합규정표준。장이상삼충역묘분별면역21일령SPF계,0.3mL/지,재면역후적일주、량주、삼주、사주,매조추취10지분별채혈,분리혈청;18일령단계0.3mL/지,재면역후적일주、량주、삼주,매조추취10지분별채혈,분리혈청,21d후이면0.5mL/지,이면단계재면역후적량주、삼주、사주、량개월、삼개월매조추취10지분별채혈,분리혈청;매차련동대조조5지혈청용금류감병독H5아형(Re-6주)항원측정HI항체,시험결과표명,미국진구백유좌제제비적중조금류감병독멸활역묘(H5N1아형, Re-6주)면역계군후,HI항체효개최고,면역지속일단시간후,효개잉보지교고,동시주안전검험대비시험,각기육주사역묘2.0mL/지,련속관찰14d,시험결과표명,이상삼충좌제제비적역묘균안전무부반응。
Avian influenza virus(AIV) was the causative agent of avian influenza(AI). The highly pathogenic H5N1 subtype AIV had the potential to infect human and sometimes is lethal. Particularly such virus can cause the fowl dead to a large extent and influence the fowl industry severely. Vaccination had been an important measure against avian influenza outbreaking. Experts believe that choice of vaccine adjuvant quality is the key to the success of the new vaccine. This test will be the same batch of avian flu (H5N1 subtype, Re-6 strain) antigen respectively with domestic white oil adjuvants, France imported white oil adjuvant and the United States imported white oil adjuvants prepara-tion into three kinds of adjuvants restructuring of recombination avian influenza vaccine, inactivated H5N1 subtype, Re-6 strain, physical properties, sterile inspection, shall conform to the prescribed standards. Will the above three kinds of vaccine SPF chicken immune to 21 age respectively, 0.3mL/each, in a week, two weeks, three weeks after immunization, around, extraction of 10 in each group respectively blood, serum separation;0.3mL/each are only 18 days of age, in a week, two weeks, three weeks after immunization, extraction of 10 in each group respectively blood, serum separation, two 0.5mL/each free after 21d, two free of laying hens in two weeks, three weeks, four weeks after immunization, two months, three months from 10 in each group respectively blood, serum separation;Every time to-gether with the control group only 5 serum with the H5 virus subtype (Re-6 strain) antigen determination of HI an-tibody, test results show that the United States imports of shortening the restructuring of recombination avian in-fluenza vaccine, inactivated H5N1 subtype, Re-6 strain immune from the crowd, the HI antibody titer, highest im-mune continued after a period of time, still maintaining high titer, do safety inspection comparison test at the same time, the intramuscular vaccineonly 2.0mL/each, continuous observation of 14d, the test results show that the preparation of the above three kinds of adjuvants vaccines are safe without side effects.