河南农业科学
河南農業科學
하남농업과학
JOURNAL OF HENAN AGRICULTURAL SCIENCES
2013年
12期
129-132
,共4页
魏星灿%贾青%陶隽%胡慧艳
魏星燦%賈青%陶雋%鬍慧豔
위성찬%가청%도준%호혜염
猪%大麻素1型受体基因%生物信息学
豬%大痳素1型受體基因%生物信息學
저%대마소1형수체기인%생물신식학
swine%cannabinoid receptor 1 gene%bioinformatics
运用生物信息学方法分析了猪和其他21个物种 CB1基因CDs序列的系统进化关系和猪C B1基因编码蛋白质的理化性质与结构。结果显示,C B1基因同源性较高,且在进化中受到纯化选择的作用。猪CB1蛋白为疏水性跨膜蛋白,包含472个氨基酸残基,不含信号肽。其一级结构含有23个磷酸化位点、6个糖基化位点;二级结构含有47.67%的α螺旋、39.62%的无规则卷曲、12.71%的延长链;三级结构由7个α螺旋和无规则卷曲组成。研究结果表明,C B1基因可能是哺乳动物的看家基因,7条相连的α螺旋结构是猪CB1的活性位点。
運用生物信息學方法分析瞭豬和其他21箇物種 CB1基因CDs序列的繫統進化關繫和豬C B1基因編碼蛋白質的理化性質與結構。結果顯示,C B1基因同源性較高,且在進化中受到純化選擇的作用。豬CB1蛋白為疏水性跨膜蛋白,包含472箇氨基痠殘基,不含信號肽。其一級結構含有23箇燐痠化位點、6箇糖基化位點;二級結構含有47.67%的α螺鏇、39.62%的無規則捲麯、12.71%的延長鏈;三級結構由7箇α螺鏇和無規則捲麯組成。研究結果錶明,C B1基因可能是哺乳動物的看傢基因,7條相連的α螺鏇結構是豬CB1的活性位點。
운용생물신식학방법분석료저화기타21개물충 CB1기인CDs서렬적계통진화관계화저C B1기인편마단백질적이화성질여결구。결과현시,C B1기인동원성교고,차재진화중수도순화선택적작용。저CB1단백위소수성과막단백,포함472개안기산잔기,불함신호태。기일급결구함유23개린산화위점、6개당기화위점;이급결구함유47.67%적α라선、39.62%적무규칙권곡、12.71%적연장련;삼급결구유7개α라선화무규칙권곡조성。연구결과표명,C B1기인가능시포유동물적간가기인,7조상련적α라선결구시저CB1적활성위점。
In the study ,the phylogenetic relationship of the coding sequences (CDS) of CB1 gene between swine and other 21 species ,and the physicochemical characters and structural properties of CB1-encoding protein in swine were analyzed with bioinformatics methods .The results showed that the homology of CB1 gene was high as purifying selection could exist in its evolution .The CB1 protein was a hydrophobic transmembrane protein consisting of 472 amino acid residues without signal peptide .The primary structure of the protein CB1 contained 23 phosphorylation sites and 6 glycosylation sites ,the secondary structure was made up of 47 .67% of α-helix , 39 .62% of random coil ,12 .71% of extended strand ,the tertiary structure was composed of 7α-heli-ces and random coil .The results indicate that CB1 maybe is a housekeeping gene of mammals and the 7 connected α-helices are active sites of CB1 in swine .