CAJ | 학술논문

根据刺五加法呢基焦磷酸合酶（farnesyl diphosphate synthase ，FPS）基因的cDNA 序列信息，设计特异性引物，利用半定量RT PCR技术，对刺五加 FPS基因在不同产地、不同器官、不同发育时期及茉莉酸甲酯（MeJA）处理后的表达情况进行分析。结果表明，FPS基因在不同产地、时期、器官及MeJA处理后的刺五加中均有表达，但表达量存在显著差异。 FPS在萌芽期的表达量最高，盛花期的表达量最低，两者比值为4．19且差异显著；本溪、珲春、鸡西、雾灵山、穆棱、梅河口、伊通产地刺五加的 FPS相对表达量依次降低；在不同器官中以叶的表达量最高；MeJA 处理后FPS的表达量比用蒸馏水处理的对照组均有显著提高。 FPS的相对表达量与刺五加叶器官的皂苷含量存在显著的正相关关系。
근거자오가법니기초린산합매（farnesyl diphosphate synthase ，FPS）기인적cDNA 서렬신식，설계특이성인물，이용반정량RT PCR기술，대자오가 FPS기인재불동산지、불동기관、불동발육시기급말리산갑지（MeJA）처리후적표체정황진행분석。결과표명，FPS기인재불동산지、시기、기관급MeJA처리후적자오가중균유표체，단표체량존재현저차이。 FPS재맹아기적표체량최고，성화기적표체량최저，량자비치위4．19차차이현저；본계、혼춘、계서、무령산、목릉、매하구、이통산지자오가적 FPS상대표체량의차강저；재불동기관중이협적표체량최고；MeJA 처리후FPS적표체량비용증류수처리적대조조균유현저제고。 FPS적상대표체량여자오가협기관적조감함량존재현저적정상관관계。
Primers were designed according to the available sequence information of the cloned FPS(farnesyl diphosphate synthase ) gene of Eleutherococcus senticosus .Semi-quantitative RT-PCR was developed to analyze relative expression quantity of FPS gene during different grow th , different dates ,different organs ,and the organs treated with MeJA .The results showed that FPS gene was expressed but with significant difference at expression levels .FPS had a highest expres-sion level during the germination stage and the lowest level during the full flowering stage .The ratio of the highest level to the lowest level was 4 .19 ,with a significant difference .FPS relative expression reduced in order of the individuals from Benxi ,Hunchun ,Jixi ,Wulingshan Mountain , Muling ,Meihekou ,and Yitong .The highest expression value of FPS was in leaf blade .After trea-ted with MeJA ,FPS relative expression increased and was higher than that of the control group . There was significantly positive correlation between the content of Eleutherococcus senticosus sap-onins and the expression levels of FPS gene .