中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2013年
4期
486-489
,共4页
HSP70热休克蛋白质类/遗传学%HSP90热休克蛋白质类/遗传学%前体细胞淋巴母细胞白血病淋巴瘤%糖皮质激素类
HSP70熱休剋蛋白質類/遺傳學%HSP90熱休剋蛋白質類/遺傳學%前體細胞淋巴母細胞白血病淋巴瘤%糖皮質激素類
HSP70열휴극단백질류/유전학%HSP90열휴극단백질류/유전학%전체세포림파모세포백혈병림파류%당피질격소류
HSP70 heat-shock proteins/genetics%HSP90 heat-shock proteins/genetics%Precursor cell lymphoblastic leukemia-lymphoma%Glucocorticoids
目的 探讨HSP70、HSP90基因表达与急性淋巴细胞白血病(ALL)糖皮质激素抵抗关系.方法 初诊急性淋巴细胞白血病患者43例,均接受泼尼松治疗.根据疗效分为糖皮质激素敏感(GCS)组(28例)和糖皮质激素抵抗(GCR)组(15例);以16名健康志愿者为正常对照组.采用实时荧光定量PCR方法检测HSP70 mRNA、HSP90 mRNA在骨髓单个核细胞(BMMNCs)中的表达.分析患者组与正常组、GCS组和GCR组、肿瘤重度负荷组和肿瘤轻度负荷组的组间表达差异.结果 (1)ALL患者组HSP70 mRNA(0.95 ±0.36)、HSP90 mRNA(1.97±0.63)的表达均明显高于正常对照组(0.28 ±0.17,0.62 ±0.21,P＜0.01);(2) GCR组的HSP70 mRNA(1.09±0.22)、HSP90 mRNA(2.18±0.42)的表达均明显高于GCS组(0.57 ±0.19,1.43±0.35,P＜0.05,P＜0.01);(3)肿瘤重度负荷组的HSP70 mRNA (0.97±0.34)、HSP90 mRNA(2.01±0.58)的表达均明显高于肿瘤轻度负荷组(0.61 ±0.22,1.34±0.29,P＜0.01).结论 ALL糖皮质激素抵抗患者存在HSP90及HSP70过表达;检测HSP90,HSP70的表达,对于ALL患者预后判定,化疗方案选择及疗效判定方面具有参考意义.
目的 探討HSP70、HSP90基因錶達與急性淋巴細胞白血病(ALL)糖皮質激素牴抗關繫.方法 初診急性淋巴細胞白血病患者43例,均接受潑尼鬆治療.根據療效分為糖皮質激素敏感(GCS)組(28例)和糖皮質激素牴抗(GCR)組(15例);以16名健康誌願者為正常對照組.採用實時熒光定量PCR方法檢測HSP70 mRNA、HSP90 mRNA在骨髓單箇覈細胞(BMMNCs)中的錶達.分析患者組與正常組、GCS組和GCR組、腫瘤重度負荷組和腫瘤輕度負荷組的組間錶達差異.結果 (1)ALL患者組HSP70 mRNA(0.95 ±0.36)、HSP90 mRNA(1.97±0.63)的錶達均明顯高于正常對照組(0.28 ±0.17,0.62 ±0.21,P＜0.01);(2) GCR組的HSP70 mRNA(1.09±0.22)、HSP90 mRNA(2.18±0.42)的錶達均明顯高于GCS組(0.57 ±0.19,1.43±0.35,P＜0.05,P＜0.01);(3)腫瘤重度負荷組的HSP70 mRNA (0.97±0.34)、HSP90 mRNA(2.01±0.58)的錶達均明顯高于腫瘤輕度負荷組(0.61 ±0.22,1.34±0.29,P＜0.01).結論 ALL糖皮質激素牴抗患者存在HSP90及HSP70過錶達;檢測HSP90,HSP70的錶達,對于ALL患者預後判定,化療方案選擇及療效判定方麵具有參攷意義.
목적 탐토HSP70、HSP90기인표체여급성림파세포백혈병(ALL)당피질격소저항관계.방법 초진급성림파세포백혈병환자43례,균접수발니송치료.근거료효분위당피질격소민감(GCS)조(28례)화당피질격소저항(GCR)조(15례);이16명건강지원자위정상대조조.채용실시형광정량PCR방법검측HSP70 mRNA、HSP90 mRNA재골수단개핵세포(BMMNCs)중적표체.분석환자조여정상조、GCS조화GCR조、종류중도부하조화종류경도부하조적조간표체차이.결과 (1)ALL환자조HSP70 mRNA(0.95 ±0.36)、HSP90 mRNA(1.97±0.63)적표체균명현고우정상대조조(0.28 ±0.17,0.62 ±0.21,P＜0.01);(2) GCR조적HSP70 mRNA(1.09±0.22)、HSP90 mRNA(2.18±0.42)적표체균명현고우GCS조(0.57 ±0.19,1.43±0.35,P＜0.05,P＜0.01);(3)종류중도부하조적HSP70 mRNA (0.97±0.34)、HSP90 mRNA(2.01±0.58)적표체균명현고우종류경도부하조(0.61 ±0.22,1.34±0.29,P＜0.01).결론 ALL당피질격소저항환자존재HSP90급HSP70과표체;검측HSP90,HSP70적표체,대우ALL환자예후판정,화료방안선택급료효판정방면구유삼고의의.
Objectives To investigate the relationships of the expressions of heat shock protein 70(HSP70) and HSP90 genes in bone marrow mononuclear cells (BMMNCs) with glucocorticoid (GC)-resistence (GCR) in acute lymphoblastic leukemia (ALL) patients.Methods The expressions of HSP70 and HSP90 in bone marrow mononuclear cells from 43 newly diagnosed ALL patients and 16 healthy volunteers were examined by real time polymerase chain reaction.Of them,43 patients received glucocorticoid therapy were divided into GC-sensitive (GCS) group and GC-resistant(GCR) group according to GC response.Results (1)The expressions of HSP70 mRNA (0.95 ± 0.36) and HSP90 mRNA (1.97 ± 0.63) in BMMNCs from ALL patients were significantly higher than that in the controls [(0.28 ±0.17)and (0.62 ±0.21),P＜0.01]; (2)The expressions of HSP70 mRNA(1.09 ± 0.22)and HSP90 mRNA(2.18 ± 0.42) in BMMNCs from GCR group patients were significantly higher than that in the GCS group patients [(0.57 ±0.19)and (1.43 ± 0.35),P ＜ 0.05,P ＜ 0.01] ; (3)The expressions of HSP70 mRNA (0.97 ± 0.34)and HSP90 mRNA (2.01 ± 0.58) in BMMNCs from the ALL patients with high-degree tumor burden were significantly higher than that in the patients with low-degree tumor burden [(0.61 ±0.22) and (1.34 ±0.29),P ＜0.01].Conclusions The mRNA expressions of HSP70 and HSP90 in BMMNCs from GCR group patients were significantly increased.Detection of HSP90 and HSP70 expressions may help ALL patients for prognosis judgment,chemotherapy plan selection and therapeutic effect evaluation.
